Recombinant Human Pro-GABARAPL1 Protein, CF
Recombinant Human Pro-GABARAPL1 Protein, CF Summary
pro-GABARAPL1 is an immature form of the protein that includes C-terminal Lys 117. This form is not competent for in vitro conjugation reactions as supplied. This protein may be used as a negative control for conjugation reactions, or as a substrate for the processing enzyme ATG4B.
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (X μM) in 50 mM HEPES, pH 7.5
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Pro-gamma -Aminobutyric Acid Receptor-associated Protein-like 1 (GABARAPL1) is a 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins. GABARAPL1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAPL1 was first described as the human ortholog of the guinea pig gec1 gene product, and is now known for its role in autophagy. Mature GABARAPL1 (in which Lys 117 has been cleaved off by the ATG4B processing enzyme) covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. GABARAPL1 is involved in the late stages of autophagosome maturation, and may also be involved in cargo recruitment to autophagosomes.
Le Grand, J.N. et al. (2011) Autophagy 7:1098
Shpilka, T. et al. (2011) Genome Biol. 12:226.
Vernier-Magnin, S. et al. (2001) Biochem. Biophy. Res. Com. 284:118.
Weidberg, H. et al. (2010) EMBO J. 29:1792.
Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
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