Pro-gamma -Aminobutyric Acid Receptor-associated Protein-like 1 (GABARAPL1) is a 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins. GABARAPL1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAPL1 was first described as the human ortholog of the guinea pig gec1 gene product, and is now known for its role in autophagy. Mature GABARAPL1 (in which Lys 117 has been cleaved off by the ATG4B processing enzyme) covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. GABARAPL1 is involved in the late stages of autophagosome maturation, and may also be involved in cargo recruitment to autophagosomes.
Recombinant Human Pro-GABARAPL1 Protein, CF
R&D Systems | Catalog # UL-400
Key Product Details
Source
Accession Number
Applications
Product Specifications
Source
Purity
Predicted Molecular Mass
Activity
pro-GABARAPL1 is an immature form of the protein that includes C-terminal Lys 117. This form is not competent for in vitro conjugation reactions as supplied. This protein may be used as a negative control for conjugation reactions, or as a substrate for the processing enzyme ATG4B.
Formulation, Preparation, and Storage
UL-400
| Formulation | X mg/ml (X μM) in 50 mM HEPES, pH 7.5 |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GABARAPL1
References
-
Le Grand, J.N. et al. (2011) Autophagy 7:1098
-
Shpilka, T. et al. (2011) Genome Biol. 12:226.
-
Vernier-Magnin, S. et al. (2001) Biochem. Biophy. Res. Com. 284:118.
-
Weidberg, H. et al. (2010) EMBO J. 29:1792.
-
Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GABARAPL1 Products
Product Documents for Recombinant Human Pro-GABARAPL1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Pro-GABARAPL1 Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human Pro-GABARAPL1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Pro-GABARAPL1 Protein, CF and earn rewards!
Have you used Recombinant Human Pro-GABARAPL1 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review