Recombinant Human Procalcitonin Protein, CF

Catalog # Availability Size / Price Qty
9607-PN-050
The Innovator Series.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human Procalcitonin Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
E. coli-derived human Procalcitonin protein
Pro27-Asn141, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Pro27
Predicted Molecular Mass
14 kDa
SDS-PAGE
14 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9607-PN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Procalcitonin

Procalcitonin (PCT) is encoded by a single CALCA gene, and can result in 2 alternatively spliced products: calcitonin and calcitonin-gene related peptide (CGRP) (1). Mature PCT is expressed as a 116 amino acid (aa) protein which is subsequently cleaved into 3 parts: a 57 aa pro-region, a 32 aa Calcitonin peptide and a 21 aa Katacalcin peptide (2, 3). PCT is moderately conserved among species, with human sharing 72% and 75% sequence identity with mouse and rat, respectively. In healthy adults, PCT is expressed by thyroid C cells and promptly converted to calcitonin (3). Calcitonin is directly involved in osteoclast activity and bone resorption (4). However, in states of inflammatory disease or bacterial infection, PCT is over-expressed from multiple cell types and the levels of unprocessed PCT rise significantly (3-5). These increased levels of PCT in circulation have been used as a diagnostic marker of systemic bacterial infection and sepsis and as a guide to antibiotic therapy (5, 6). More recently, the use of PCT as a disease marker has been expanded to include acute heart failure and vasculitic disorders (7, 8).

References
  1. Russwurm, S. et al. (2005). Clin Chem and Lab Med, 37:789.
  2. Le Moullec, J.M. et al. (1984) FEBS Lett 167:93.
  3. Maruna P. et al. (2000) Physiol Res;49 Suppl 1:S57.
  4. Becker, KL et al. (2004). J Clin Endocrinol Metab 89:1512.
  5. Müller B. et al. (2001) Jan;86(1):396-404. J Clin Endocrinol Metab.
  6. Meisner, M. et al. (2014) Ann Lab Med. Jul; 34:263.
  7. Möckel, M. et al. (2017) ESC Heart Failure, 4:203.
  8. Lee NH, et al. (2017) Korean J of Peds 60:112.
Entrez Gene IDs
796 (Human); 12310 (Mouse); 24241 (Rat)
Alternate Names
CALC1; CALCA; Calcitonin 1; calcitonin gene-related peptide 1; CGRP; Katacalcin; Procalcitonin

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human Procalcitonin Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Procalcitonin Protein, CF and earn rewards!

Have you used Recombinant Human Procalcitonin Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review