Recombinant Human Procalcitonin Protein, CF Summary
Pro27-Asn141, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.
Procalcitonin (PCT) is encoded by a single CALCA gene, and can result in 2 alternatively spliced products: calcitonin and calcitonin-gene related peptide (CGRP) (1). Mature PCT is expressed as a 116 amino acid (aa) protein which is subsequently cleaved into 3 parts: a 57 aa pro-region, a 32 aa Calcitonin peptide and a 21 aa Katacalcin peptide (2, 3). PCT is moderately conserved among species, with human sharing 72% and 75% sequence identity with mouse and rat, respectively. In healthy adults, PCT is expressed by thyroid C cells and promptly converted to calcitonin (3). Calcitonin is directly involved in osteoclast activity and bone resorption (4). However, in states of inflammatory disease or bacterial infection, PCT is over-expressed from multiple cell types and the levels of unprocessed PCT rise significantly (3-5). These increased levels of PCT in circulation have been used as a diagnostic marker of systemic bacterial infection and sepsis and as a guide to antibiotic therapy (5, 6). More recently, the use of PCT as a disease marker has been expanded to include acute heart failure and vasculitic disorders (7, 8).
- Russwurm, S. et al. (2005). Clin Chem and Lab Med, 37:789.
- Le Moullec, J.M. et al. (1984) FEBS Lett 167:93.
- Maruna P. et al. (2000) Physiol Res;49 Suppl 1:S57.
- Becker, KL et al. (2004). J Clin Endocrinol Metab 89:1512.
- Müller B. et al. (2001) Jan;86(1):396-404. J Clin Endocrinol Metab.
- Meisner, M. et al. (2014) Ann Lab Med. Jul; 34:263.
- Möckel, M. et al. (2017) ESC Heart Failure, 4:203.
- Lee NH, et al. (2017) Korean J of Peds 60:112.
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