Recombinant Human Prostatic Acid Phosphatase/ACPP, CF
R&D Systems | Catalog # 6240-AP
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Key Product Details
- R&D Systems NS0-derived Recombinant Human Prostatic Acid Phosphatase/ACPP (6240-AP)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Prostatic Acid Phosphatase/ACPP protein
Met1-Gln379, with a C-terminal 6-His tag
Met1-Gln379, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys33
Predicted Molecular Mass
41 kDa
SDS-PAGE
43-55 kDa, reducing conditions
Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >100,000 pmol/min/ug, as measured under the described conditions.
The specific activity is >100,000 pmol/min/ug, as measured under the described conditions.
Formulation, Preparation, and Storage
6240-AP
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Do not freeze.
|
Background: Prostatic Acid Phosphatase/ACPP
References
- Apostol, I. et al. (1985) Acta. Biochim. Pol. 32:187.
- Schroeder, B. et al. (2007) Traffic 8:1676.
- Gutman, E.B. et al. (1936) Am. J. Cancer 28:485.
- Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351.
- Veeramani, S. et al. (2005) Endocr. Relat. Cancer 12:805.
Alternate Names
ACP3, ACPP
Gene Symbol
ACP3
UniProt
Additional Prostatic Acid Phosphatase/ACPP Products
Product Documents for Recombinant Human Prostatic Acid Phosphatase/ACPP, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Prostatic Acid Phosphatase/ACPP, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human Prostatic Acid Phosphatase/ACPP, CF (6240-AP):
Materials
- Assay Buffer: 50 mM NaOAc, pH 4.5
- Recombinant Human Prostatic Acid Phosphatase/ACPP (rhACPP) (Catalog # 6240-AP)
- Substrate: p-Nitrophenyl phosphate (Sigma-Aldrich, Catalog # N2765)
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent]
- NaOH, 0.2 M in deionized water
- Dilute rhACPP to 0.1 μg/mL in Assay Buffer.
- Dilute Substrate to 2 mM in Assay Buffer.
- In a plate combine 50 μL of rhACPP and 50 μL of 2 mM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer and 50 μL of 2 mM Substrate.
- Incubate reaction at room temperature for 5 minutes.
- Add 100 μL of 0.2 M NaOH to stop the reaction and develop the color.
- Read (top read) absorbance in endpoint mode at 410 nm.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Abs* (OD) x Conversion Factor** (pmol/OD) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard p-Nitrophenol (Sigma-Aldrich, Catalog # 241326).
- rhACPP: 0.005 μg
- Substrate: 0.5 mM