Recombinant Human Prostatic Acid Phosphatase/ACPP, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details

Recombinant Human Prostatic Acid Phosphatase/ACPP, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/ug, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Prostatic Acid Phosphatase/ACPP protein
Met1-Gln379, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
41 kDa
43-55 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM NaOAc, pH 4.5
  • Recombinant Human Prostatic Acid Phosphatase/ACPP (rhACPP) (Catalog # 6240-AP)
  • Substrate: p-Nitrophenyl phosphate (Sigma-Aldrich, Catalog # N2765)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent]
  • NaOH, 0.2 M in deionized water
  1. Dilute rhACPP to 0.1 μg/mL in Assay Buffer.
  2. Dilute Substrate to 2 mM in Assay Buffer.
  3. In a plate combine 50 μL of rhACPP and 50 μL of 2 mM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer and 50 μL of 2 mM Substrate.
  4. Incubate reaction at room temperature for 5 minutes.
  5. Add 100 μL of 0.2 M NaOH to stop the reaction and develop the color.
  6. Read (top read) absorbance in endpoint mode at 410 nm.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard p-Nitrophenol (Sigma-Aldrich, Catalog # 241326).

Per Well:
  • rhACPP: 0.005 μg
  • Substrate: 0.5 mM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Prostatic Acid Phosphatase/ACPP

Prostatic Acid Phosphatase (ACPP) catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins (1). The activity optimum of ACPP is in the pH range of 4 - 6, and the activity is inhibited by L(+)-tartrate. ACPP expression levels are highest in the prostate, with much lower expression in most other tissues. ACPP is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists (2). The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer (3). Cellular ACPP has been shown to be a protein tyrosine phosphatase (4). Protein substrates include the epidermal growth factor receptor and HER-2 (5). Cellular ACPP is considered to function as a tumor suppressor (5).

  1. Apostol, I. et al. (1985) Acta. Biochim. Pol. 32:187.
  2. Schroeder, B. et al. (2007) Traffic 8:1676.
  3. Gutman, E.B. et al. (1936) Am. J. Cancer 28:485.
  4. Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351.
  5. Veeramani, S. et al. (2005) Endocr. Relat. Cancer 12:805.
Entrez Gene IDs
55 (Human); 563181 (Mouse); 56780 (Rat)
Alternate Names
acid phosphatase, prostate; ACP3; ACP-3; ACPP; EC; PAP; Prostatic Acid Phosphatase; prostatic acid phosphotase


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