Recombinant Human Protocadherin gamma C3 Protein, CF Summary
Ser30-Tyr693, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Protocadherin gamma C3
Protocadherin gamma C3 is a member of the gamma subgroup of clustered protocadherins (1). Like other gamma protocadherins, mature Protocadherin gamma C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin gamma C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA2+ -dependent homophilic interaction (4). MMP-mediated shedding of gamma protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin gamma C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma gene cluster is embryonic lethal in mice (9). Protocadherin gamma C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin gamma gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma gene cluster results in dendritic arborization defects (16). The protocadherin gamma subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).
- Yagi, T. (2008) Dev. Growth Differ. 50:S131.
- Sano, K. et al. (1993) EMBO J. 12:2249.
- Kohmura, N. et al. (1998) Neuron 20:1137.
- Obata, S. et al. (1995) J. Cell Sci. 108:3765.
- Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.
- Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.
- Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
- Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.
- Wang, X. et al. (2002) Neuron 36:843.
- Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.
- Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.
- Lefebvre, J.L. et al. (2008) Development 135:4141.
- Prasad, T. et al. (2008) Development 135:4153.
- Lin, C. et al. (2010) J. Biol. Chem. 285:41675.
- Chen, W.V. et al. (2012) Neuron 75:402.
- Garrett, A.M. et al. (2012) Neuron 74:269.
- Lobas, M.A. et al. (2012) J. Neurochem. 120:913.
- Ledderose, J. et al. (2013) Sci. Rep. 3:1514.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Protocadherin gamma C3 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Protocadherin gamma C3 Protein, CF and earn rewards!
Have you used Recombinant Human Protocadherin gamma C3 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image