Recombinant Human PVRIG Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9365-PV-050
Recombinant Human PVRIG Fc Chimera Protein Bioactivity
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Recombinant Human PVRIG Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PVRIG Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human
Nectin-2/CD112 (Catalog # 2229-N2) that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human PVRIG protein
Human PVRIG
(Thr41 & Glu43-Leu172)
Accession # Q6DKI7
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr41 & Glu43
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa
SDS-PAGE
51-57 kDa, reducing conditions

Product Datasheets

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9365-PV

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9365-PV

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and PEG with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human PVRIG Fc Chimera Protein Bioactivity View Larger

When Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds with an ED50 of 0.4-2.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

References
  1. Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.
Long Name
Poliovirus Receptor Related Immunoglobulin Domain Containing
Entrez Gene IDs
79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)
Alternate Names
C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; poliovirus receptor related immunoglobulin domain containing; Poliovirus receptor-related immunoglobulin domain-containing protein; PVRIG; transmembrane protein PVRIG

Citation for Recombinant Human PVRIG Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022-03-25;13(1):1606.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Flow Cytometry

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