Recombinant Human Reg1A Protein, CF

Catalog # Availability Size / Price Qty
9745-RE-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Reg1A Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Reg1A, immobilized at 5 μg/mL, is able to significantly enhance neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Reg1A protein
Gln23-Asn166
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln23 inferred from enzymatic pyroglutamate treatment revealing Glu24
Structure / Form

Predicted Molecular Mass
16 kDa
SDS-PAGE
17-20 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9745-RE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg1A

Reg1A (Regenerating islet-derived 1 alpha), also called Lithostatine-1-alpha or Pancreatic stone protein (PSP), is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins of the genes. Reg1A is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1A cDNA encodes a 166 amino acids (aa) precursor which contains a 22 aa signal peptide and a 144 aa secreted mature protein. Human Reg1A shares 86% aa sequence identity with Reg1B, and shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively. Reg1A was first described in cells of digestive systems, but is also expressed in a variety of other tissues including neuronal cell lines (PC12 and Neuro-2a) and in rat primary hippocampal neurons (3, 4). In the digestive system, Reg-1A acts as a paracrine/autocrine factor and plays an important role in cell proliferation, differentiation, inflammation, and carcinogenesis (5, 6). In neurons, Reg-1A is preferentially localized at the membrane and around the nucleus of neuronal cells (4). In addition, Reg-1A is secreted, and it positively regulates neurite outgrowth possibly through the membrane receptor exostosin tumor-like 3 (EXTL3) (4). Reg-1A is overexpressed during the very early stages of Alzheimer disease, and its deposits were detected in the brain of patients with Alzheimer disease (7).

References
  1. Liu, J.L. et al. (2008) Endocr. Metab. Immune. Disord. Drug Targets 8:1.
  2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
  3. De Caro A. et al. (1979) Biochem. Biophys. Res. Commun. 87:1176
  4. Acquatella-Tran Van Ba, I. et al. (2012) J. Biol. Chem. 287:4726.
  5. Zhang Y.W. et al. (2003) World J. Gastroenterol. 9:2635.
  6. Miyaoka Y. et al. (2004) Oncogene 23:3572.
  7. Duplan, L. et al. (2001) Neurobiol. Aging 22:79.
Long Name
Regenerating Islet-derived 1A
Entrez Gene IDs
5967 (Human)
Alternate Names
ICRF; Islet cells regeneration factor; Islet of Langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; Pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; Reg1A; REG-1-alpha; Regenerating islet-derived protein 1-alpha; Regenerating protein I alpha

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