Recombinant Human Reg1A Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reg1A (Regenerating islet-derived 1 alpha), also called Lithostatine-1-alpha or Pancreatic stone protein (PSP), is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins of the genes. Reg1A is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1A cDNA encodes a 166 amino acids (aa) precursor which contains a 22 aa signal peptide and a 144 aa secreted mature protein. Human Reg1A shares 86% aa sequence identity with Reg1B, and shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively. Reg1A was first described in cells of digestive systems, but is also expressed in a variety of other tissues including neuronal cell lines (PC12 and Neuro-2a) and in rat primary hippocampal neurons (3, 4). In the digestive system, Reg-1A acts as a paracrine/autocrine factor and plays an important role in cell proliferation, differentiation, inflammation, and carcinogenesis (5, 6). In neurons, Reg-1A is preferentially localized at the membrane and around the nucleus of neuronal cells (4). In addition, Reg-1A is secreted, and it positively regulates neurite outgrowth possibly through the membrane receptor exostosin tumor-like 3 (EXTL3) (4). Reg-1A is overexpressed during the very early stages of Alzheimer disease, and its deposits were detected in the brain of patients with Alzheimer disease (7).
- Liu, J.L. et al. (2008) Endocr. Metab. Immune. Disord. Drug Targets 8:1.
- Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
- De Caro A. et al. (1979) Biochem. Biophys. Res. Commun. 87:1176
- Acquatella-Tran Van Ba, I. et al. (2012) J. Biol. Chem. 287:4726.
- Zhang Y.W. et al. (2003) World J. Gastroenterol. 9:2635.
- Miyaoka Y. et al. (2004) Oncogene 23:3572.
- Duplan, L. et al. (2001) Neurobiol. Aging 22:79.
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