Reg1B also called Lithostatine-1-beta or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons (3).
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Reg1B Protein (2090-RG)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human Reg1B protein
Gln23-Asn166, with an N-terminal Met
Gln23-Asn166, with an N-terminal Met
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
16 kDa
SDS-PAGE
16 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells.
The ED50 for this effect is 0.15-0.75 μg/mL.
The ED50 for this effect is 0.15-0.75 μg/mL.
Reviewed Applications
Read 1 review rated 5 using 2090-RG in the following applications:
Scientific Data Images for Recombinant Human Reg1B Protein, CF
Recombinant Human Reg1B Protein Bioactivity
Recombinant Human Reg1B (Catalog # 2090-RG) stimulates cell proliferation in RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.15-0.75 μg/mL.
Recombinant Human Reg1B Protein SDS-PAGE
1 μg/lane of Recombinant Human Reg1B (Catalog # 2090-RG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 17.2 and 16.7 kDa, respectively.Formulation, Preparation, and Storage
2090-RG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: Reg1B
References
- Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
- Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
- Livesey F.J. et al. (1990) Nature. 390:614.
Long Name
Regenerating Islet-derived 1B
Alternate Names
Lithostathine 1 beta, PSPS2, REGH
Entrez Gene IDs
5968 (Human)
Gene Symbol
REG1B
UniProt
Additional Reg1B Products
Product Documents for Recombinant Human Reg1B Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Reg1B Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human Reg1B Protein, CF (1)
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Application: Immunoassay StandardVerified Customer | Posted 08/20/2021Combined with MAB2090 and AF2090 in an ELISA.
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