Recombinant Human Relaxin-3 Protein

R&D Systems | Catalog # 3107-RN

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Relaxin-3 Protein (3107-RN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q8WXF3

Structure / Form

Disulfide-linked heterodimer

Applications

Bioactivity
View all Relaxin-3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Relaxin-3 protein
Asp119-Cys142 (A chain) & Ala27-Arg53 (B chain)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp119 (A chain) & Ala27 (B chain)

Predicted Molecular Mass

2.46 kDa (A chain), 3.04 kDa (B chain)

Activity

Measured by its ability to induce cAMP accumulation in THP‑1 human acute monocytic leukemia cells. Parsell, D.A. et al. (1996) J. Biol. Chem. 271:27936.
The ED50 for this effect is 3.5‑17.5 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3107-RN
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 3107-RN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Relaxin-3

Human Relaxin-3 (H3 relaxin, INSL7) is one of seven relaxin-like peptides belonging to the insulin superfamily (1 - 4). Unlike human relaxins 1 and 2, it does not play a role in reproduction but appears to be a neuropeptide involved in stress response in the brain stem (3 - 5). The 142 amino acid (aa) Relaxin-3 pre-proprotein is processed to remove a 25 aa signal peptide and a connecting peptide (aa 53 - 118). The resulting mature Relaxin-3 is a 5.5 kDa, 51 aa secreted heterodimer of A (aa 119 - 142) and B (aa 26 - 52) peptides connected by two intermolecular disulfide bonds (1). Mature human Relaxin-3 is 96%, 94%, and 92% aa identical to porcine, canine, and mouse Relaxin-3, respectively. This is much higher identity between species than that seen for other relaxins. Relaxin-3 is thus suggested to be the ancestral relaxin family member (2). Relaxin-3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated RXFP3 (4, 6). In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular nucleus (6). This region has connections to the dorsal tegmental region of the pons (also called the nucleus incertus), where expression of Relaxin-3 is highest (5). Relaxin-3 also binds the more widely-expressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin-2 (1, 7). Although binding of Relaxin-3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits cAMP accumulation, indicating coupling to Gi, Go or Gz by this receptor (1, 5). Relaxin-3 expression does not overlap well with its other receptor, GPCR142, which instead appears to be the primary receptor for INSL5 (3, 8).

References

  1. Kizawa, H. et al. (2003) Regul. Pept. 113:79.
  2. Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14.
  3. Bathgate, R.A. et al. (2006) Pharmacol. Rev. 58:7.
  4. Liu, C. et al. (2005) Ann. N.Y. Acad. Sci. 1041:47.
  5. Tanaka, M. et al. (2005) Eur. J. Neurosci. 21:1659.
  6. Liu, C. et al. (2003) J. Biol. Chem. 278:50754.
  7. Rosengren, K.J. et al. (2006) J. Biol. Chem. 281:5845.
  8. Liu, C. et al. (2003) J. Biol. Chem. 278:50765.

Alternate Names

H3, INSL7, M3, Relaxin3, RLN3, RXN3, ZINS4

Entrez Gene IDs

117579 (Human)

Gene Symbol

RLN3

UniProt

Q8WXF3

Additional Relaxin-3 Products

Product Documents for Recombinant Human Relaxin-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 3107-RN (with carrier)
Download SDS 3107-RN/CF (carrier free)

Product Specific Notices for Recombinant Human Relaxin-3 Protein

For research use only

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