Recombinant Human Relaxin R1 Fc Chimera Protein, CF

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Recombinant Human Relaxin R1 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When biotinylated Recombinant Human Relaxin-2 is added to serially diluted Recombinant Human Relaxin R1 Fc Chimera, the concentration of Recombinant Human Relaxin R1 Fc Chimera that produces 50% of the optimal binding response is typically 1.5-7.5 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human Relaxin R1 protein
Human Relaxin R1
Accession # Q9HBX9
N-terminus C-terminus
Accession #
N-terminal Sequence
Amino acid sequencing was blocked, suggesting it is consistent with Gln23 as the first N-terminal amino acid
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
69.8 kDa (monomer)
95-115 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Relaxin R1

Relaxin R1 (Relaxin receptor 1), also known as RXFP1 (Relaxin family peptide receptor 1) or LGR7 (leucine‑rich G‑protein‑coupled receptor 7) is a member of family C of the LGRs, and is one of four receptors for Relaxin family proteins. Relaxin R1 shows highest affinity for human Relaxins 1, 2 and 3, while RXFP2 binds Relaxin 2 and the related INSL3, and RXFP3 primarily binds Relaxin 3 (1, 2). The 757 amino acid (aa) human Relaxin R1 contains an N‑terminal 409 aa extracellular domain (ECD) with a calcium‑binding LDL R class A (LDLa) domain and 10 leucine‑rich repeats (LRR) with several N‑glycosylation sites. The C‑terminus contains 12 transmembrane domains within aa 410‑672. Human Relaxin R1 (aa 1‑398) shares 84, 86, 85, 85 and 91% aa sequence identity with mouse, rat, equine, bovine and porcine Relaxin R1, respectively. Isoforms of 724 and 709 aa lack aa 63‑96 and 300‑348, respectively, while isoforms of 176, 189, 191 and 337 aa diverge after aa 154, 179, 181 and 324, respectively (3, 4). These forms may dimerize with full‑length Relaxin R1 and reduce its expression on the cell surface (3, 4). Receptor activation and cAMP signaling depend on the LDLa domain, and Relaxin binding requires the LRR repeats, with a secondary binding site within transmembrane region exoloops (1, 2, 5). Of LGR family members, RXFP1 and RXFP2 are unique in that they are not internalized to down‑regulate signaling, and their LDLa domains allow transmission of both G‑protein‑dependent and ‑independent signals (1, 2, 6, 7). Engagement of Relaxin R1 by Relaxin (mainly Relaxin 2 in humans) supports female reproduction by promoting uterine angiogenesis, ovarian follicle ripening, and endometrial, cervical and nipple development (8‑10). In male reproduction, Relaxin R1 acts in the prostate to enhance sperm motility (11). It reduces fibrosis in the heart, skin, lungs, liver, kidney, and reproductive tissues by combating aberrant collagen buildup (12). In the vasculature, it mediates vasodilation and decreases blood pressure. Relaxin R1 is expressed on human leukocytes and promotes adhesion, migration, and osteoclast differentiation (13, 14). Additional effects on heart, lungs, kidney and brain are reported, some of which may be species‑specific (1).

  1. van der Westhuizen, E.T. et al. (2008) Drug Discov. Today 13:640.
  2. Kong, R.C.K. et al. (2010) Mol. Cell. Endocrinol. 320:1.
  3. Muda, M. et al. (2005) Mol. Hum. Reprod. 11:591.
  4. Kern, A. et al. (2008) Endocrinology 149:1227.
  5. Hopkins, E.J. et al. (2007) J. Biol. Chem. 282:4172.
  6. Kern, A. and G.D. Bryant-Greenwood (2009) Endocrinology 150:2419.
  7. Halls, M.L. (2012) Br. J. Pharmacol. 165:1644.
  8. Kamat, A.A. et al. (2004) Endocrinology 145:4712.
  9. Krajnc-Franken, M.A. et al. (2004) Mol. Cell. Biol. 24:687.
  10. Yao, L. et al. (2008) Endocrinology 149:2072.
  11. Ferlin, A. et al. (2012) J. Androl. 33:474.
  12. Hossain, M.A. (2011) Biochemistry 50:1368.
  13. Ferlin, A. et al. (2010) Bone 46:504.
  14. Figueiredo, K.A. et al. (2006) J. Biol. Chem. 281:3030.
Long Name
Relaxin Receptor 1
Entrez Gene IDs
59350 (Human); 381489 (Mouse); 295144 (Rat)
Alternate Names
leucine-rich repeat-containing G protein-coupled receptor 7; Leucine-rich repeat-containing G-protein coupled receptor 7; LGR7; LGR7.1; LGR7.10; LGR7LGR7.2; MGC138347; MGC142177; Relaxin family peptide receptor 1; Relaxin R1; relaxin receptor 1; relaxin/insulin-like family peptide receptor 1; RelaxinR1; RXFP1; RXFPR1


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