Recombinant Human RGM-A Protein, CF

Catalog # Availability Size / Price Qty
2459-RM-050
Product Details
Citations (2)
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Recombinant Human RGM-A Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of
10-500 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human RGM-A protein
Cys48-Gly422 & Pro169-Gly422, both with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys48 & Pro169
Predicted Molecular Mass
42.4 kDa
SDS-PAGE
Multiple bands between 25-37 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2459-RM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: RGM-A

Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).

References
  1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  2. Monnier P. et al. (2002) Nature 419:392.
  3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
  7. Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.
Long Name
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse)
Alternate Names
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

Citations for Recombinant Human RGM-A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012;7(9):e46307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  2. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay

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