Recombinant Human RGM-A Protein, CF Summary
Cys48-Gly422 & Pro169-Gly422, both with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).
- Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
- Monnier P. et al. (2002) Nature 419:392.
- Niederkofler V. et al. (2004) J. Neurosci. 24:808.
- Brinks, H. et al. (2004) J. Neurosci. 24:3862.
- Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
- Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
- Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.
Citations for Recombinant Human RGM-A Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
PLoS ONE, 2012;7(9):e46307.
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
Stem Cells, 2007;25(12):3261-70.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
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