Recombinant Human RGM-B Protein, CF

Catalog # Availability Size / Price Qty
3630-RG-050
Product Details
Citations (2)
FAQs
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Recombinant Human RGM-B Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rhRGM-B with a linear range of 10-500 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human RGM-B protein
Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly87 & Pro210
Predicted Molecular Mass
13.4 kDa (N-terminus chain), 27.7 kDa (C-terminus chain)
SDS-PAGE
20 kDa and 35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3630-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 45 amino acid (aa) signal sequence, a 368 aa mature region, and a 24 aa C-terminal prosegment (3). RGM-B contains an RGD motif, two potential N-linked glycosylation sites, and an abbreviated von Willebrand factor domain. There is a potential proteolytic cleavage site within the VWF domain (4). Alternative splicing may generate isoforms of RGM-B with N-terminal extensions or truncation following the VWF domain. Mature human RGM-B shares 52% and 36% aa sequence identity with the comparable regions of RGM-A and RGM-C, respectively. It shares 98%, 92%, 92%, and 78% aa sequence identity with macaque, mouse, bovine, and chicken RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3 - 5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2 - 5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

References
  1. Monnier, P.P. et al. (2002) Nature 419:392.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
  7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
Long Name
Repulsive Guidance Molecule B
Entrez Gene IDs
285704 (Human); 68799 (Mouse)
Alternate Names
DKFZp434P228; Dragon; DRG11-responsive axonal guidance and outgrowth of neurite; RGM domain family, member B; RGMB; RGM-B

Citations for Recombinant Human RGM-B Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014;211(5):943-59.
    Species: Mouse
    Sample Types: Antibody
    Applications: ELISA (Capture)
  2. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012;7(9):e46307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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