SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.
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Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha
R&D Systems | Catalog # 350-NS
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (350-NS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived CXCL12/SDF-1 alpha protein
Lys22-Lys89
Lys22-Lys89
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys22
Predicted Molecular Mass
8.0 kDa
SDS-PAGE
7 kDa, reducing conditions.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4.
The ED50 for this effect is 0.0600-0.600 ng/mL.
The ED50 for this effect is 0.0600-0.600 ng/mL.
Reviewed Applications
Read 5 reviews rated 4.8 using 350-NS in the following applications:
Scientific Data Images for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha Bioactivity
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.0600-0.600 ng/mL.
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha SDS-PAGE
1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 350-NS
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 350-NS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CXCL12/SDF-1 alpha
References
- Tashiro, K. et al. (1993) Science 261:600.
- Bleul, C. et al. (1996) Nature 382:829.
- Oberlin, E. et al. (1996) Nature 382:833.
Alternate Names
SDF1 alpha, SDF1a
Gene Symbol
CXCL12
UniProt
Additional CXCL12/SDF-1 alpha Products
Product Documents for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha
For research use only
Related Research Areas
Citations for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha
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Customer Reviews for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (5)
4.8 out of 5
5 Customer Ratings
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Application: Cell migration/motilitySpecies: HumanVerified Customer | Posted 04/24/2025Comparison of NK cell count/µL in the control condition (R10) vs CXCL12 at 100ng/mL200.000 sorted NK cells were place in the upper chamber of a 24 well plate with transwell inserts (5µm pore size). 650µL of culture media containing 10n0g/mL of CXCL12 were placed in the lower chamber. Cell count was performed by flow cytometry using counting bead after one hour of migration at 37ºC.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/13/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 05/27/2019
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Application: Western BlotVerified Customer | Posted 03/13/2019Receptor activation by CXCL12 Ligand to screen for NFKB pathway activation by Western Blot: Treat MDA231 cells with CXCL12 to look into whether CXCR4 activation by CXCL12 induced NFkB activation by phosphorylating IkBa by Western Blot
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Application: Cell migration/motilityVerified Customer | Posted 08/09/2016
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FAQs for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha
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Q: Does Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha Protein (Catalog # 350-NS or Catalog # 350-NS/CF) contain any tags?
A: Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha Protein (Catalog # 350-NS or Catalog # 350-NS/CF) does not contain any tags. Please refer to the Source section on the product-specific page for sequence information.
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Associated Pathways
Jak/STAT Signaling Pathway
