Recombinant Human S100A7 Protein, CF

Catalog # Availability Size / Price Qty
9085-SA-050
Recombinant Human S100A7 Protein, CF Bioactivity
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Recombinant Human S100A7 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 2-10 μg/mL.
Source
E. coli-derived human S100A7 protein
Ser2-Gln101 with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Ser2 (Major) & Met1 (Minor)
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
11 kDa
SDS-PAGE
11 kDa, reducing conditions

Product Datasheets

9085-SA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Human S100A7 Protein, CF Bioactivity View Larger

Recombinant Human S100A7 (Catalog # 9085-SA) induces IL-8secretion in A431 human epithelial carcinoma cells. The ED50 forthis effect is 2-10 μg/mL.

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Background: S100A7

S100A7, also known as Psoriasin, is a 11-12 kDa member of the S100 family of EF hand calcium binding proteins (1). Human S100A7 shares 32% amino acid sequence identity with mouse S100A7A, the closest related protein in mouse (2). It is acetylated at the N-terminus and binds both calcium and zinc ions (3, 4). S100A7 is up-regulated in keratinocytes of psoriasis and atopic dermatitis lesions (5-9), as well as in epithelial cells of the tongue, eye, and female genital tract (10-12). Its up-regulation can be induced by bacterial exposure, inflammatory cytokines, or epidermal barrier disruption (3, 9, 11, 12). S100A7 supports epithelial integrity through killing E. coli by sequestration of zinc (3, 5) and through inducing the up-regulation of tight junction proteins (13). The interaction of S100A7 with RAGE promotes the migration of immune cells and the infiltration of macrophages into tumor sites (7, 14).

References
  1. Donato, R. et al. (2013) Curr. Mol. Med. 13:24.
  2. Madsen, P. et al. (1991) J. Invest. Dermatol. 97:701.
  3. Glaser, R. et al. (2005) Nat. Immunol. 6:57.
  4. Brodersen, D.E. et al. (1999) Biochemistry 38:1695.
  5. Lee, K.C. and R.L. Eckert (2007) J. Invest. Dermatol. 127:945.
  6. Anderson, K.S. et al. (2009) Br. J. Dermatol. 160:325.
  7. Wolf, R. et al. (2008) J. Immunol. 181:1499.
  8. Al-Haddad, S. et al. (1999) Am. J. Pathol. 155:2057.
  9. Glaser, R. et al. (2009) J. Invest. Dermatol. 129:641.
  10. Meyer, J.E. et al. (2008) Mucosal Immunol. 1:239.
  11. Garreis, F. et al. (2011) Invest. Opthalmol. Vis. Sci. 52:4914.
  12. Mildner, M. et al. (2010) Mucosal Immunol. 3:602.
  13. Hattori, F. et al. (2014) Br. J. Dermatol. 171:742.
  14. Nasser, M.W. et al. (2015) Cancer Res. 75:974.
Long Name
S100 Calcium Binding Protein A7
Entrez Gene IDs
6278 (Human); 192344 (Mouse)
Alternate Names
HID 5; HID5; HID-5; protein S100-A7; PSOR1; psoriasin 1; Psoriasin; S100 calcium binding protein A7 (psoriasin 1); S100 calcium binding protein A7; S100 calcium-binding protein A7 (psoriasin 1); S100 calcium-binding protein A7; S100A7; S100A7c

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