Recombinant Human S100A8/S100A9 Heterodimer Protein, CF

(1 Review)
  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. Hibino, T. et al. (2013) Cancer Res. 73:172. The ED50 for this effect is 1.5-6 μg/mL.
  • Source
    E. coli-derived Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
  • Accession #
  • N-terminal Sequence
    Analysis
    Met1 (S100A8) & Thr2 (S100A9)
  • Structure / Form
    Noncovalently-linked heterodimer
  • Predicted Molecular Mass
    11 kDa (S100A8) & 13 kDa (S100A9)
  • SDS-PAGE
    9 kDa (S100A8) & 13 kDa (S100A9), reducing conditions
8226-S8
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 500 μg/mL in  sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human S100A8/S100A9 Heterodimer (Catalog # 8226-S8) induces IL-6 secretion by A375 human melanoma cells. The ED50 for this effect is 1.5-6 μg/mL.
Background: S100A8/S100A9 Heterodimer
S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two
EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).
  • References:
    1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
    2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
    3. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
    4. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
    5. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
    6. Odink, K. et al. (1987) Nature 330:80.
    7. Dorin, J.R. et al. (1987) Nature 326:614.
    8. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
    9. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
    10. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
    11. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
    12. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
  • Long Name:
    S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
  • Entrez Gene IDs:
    6279 (Human)
  • Alternate Names:
    Calprotectin; S100A8/S100A9 Heterodimer
Related Research Areas
Average Rating: 5 (Based on 1 review)

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Immunoassay Standard Recombinant Human S100A8/S100A9 Heterodimer Protein, CF 8226-S8
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Immunoassay Standard Recombinant Human S100A8/S100A9 Heterodimer Protein, CF 8226-S8
Immunoassay Standard: Recombinant Human S100A8/S100A9 Heterodimer Protein, CF [8226-S8]

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