Recombinant Human S100A8/S100A9 Heterodimer Protein, CF

Catalog # Availability Size / Price Qty
8226-S8-050
Recombinant Human S100A8/S100A9 Heterodimer Protein, CF Data
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Product Details
Citations (4)
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Recombinant Human S100A8/S100A9 Heterodimer Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. Hibino, T. et al. (2013) Cancer Res. 73:172. The ED50 for this effect is 1.5-6 μg/mL.
Source
E. coli-derived human S100A8/S100A9 Heterodimer protein
Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
N-terminal Sequence
Analysis
Met1 (S100A8) & Thr2 (S100A9)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
11 kDa (S100A8) & 13 kDa (S100A9)
SDS-PAGE
9 kDa (S100A8) & 13 kDa (S100A9), reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8226-S8

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 500 μg/mL in  sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Human S100A8/S100A9 Heterodimer (Catalog # 8226-S8) induces IL-6 secretion by A375 human melanoma cells. The ED50for this effect is 1.5-6 μg/mL.

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Background: S100A8/S100A9 Heterodimer

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two
EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).

References
  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
  3. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  4. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  5. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
  6. Odink, K. et al. (1987) Nature 330:80.
  7. Dorin, J.R. et al. (1987) Nature 326:614.
  8. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
  9. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
  10. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
  11. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
  12. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
Long Name
S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Entrez Gene IDs
6279 (Human)
Alternate Names
Calprotectin; S100A8/S100A9 Heterodimer

Citations for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Platelets Fuel the Inflammasome Activation of Innate Immune Cells
    Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
    Cell Rep, 2020;31(6):107615.
    Species: Human
    Sample Types:
    Applications: Cell Culture
  2. High plasma level of S100A8/S100A9 and S100A12 at admission indicates a higher risk of death in septic shock patients
    Authors: C Dubois, D Marcé, V Faivre, AC Lukaszewic, C Junot, F Fenaille, S Simon, F Becher, N Morel, D Payen
    Sci Rep, 2019;9(1):15660.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  3. A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing
    Authors: A Basu, S Munir, MA Mulaw, K Singh, B Herold, D Crisan, A Sindrilaru, N Treiber, M Wlaschek, M Huber-Lang, F Gebhard, K Scharffett
    Sci Rep, 2018;8(1):6205.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Necrotizing enterocolitis is associated with acute brain responses in preterm pigs
    Authors: J Sun, X Pan, LI Christians, XL Yuan, K Skovgaard, DEW Chatterton, SS Kaalund, F Gao, PT Sangild, S Pankratova
    J Neuroinflammation, 2018;15(1):180.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human S100A8/S100A9 Heterodimer Protein, CF
By Anonymous on 06/24/2016
Application: Immunoassay Standard