Recombinant Human SECTM1 Fc Chimera Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human SECTM1 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 x 104 cells per well are added to Recombinant Human SECTM1 Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 1.5-7.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived human SECTM1 protein
Human SECTM1
Accession # Q8WVN6
N-terminus C-terminus
Accession #
N-terminal Sequence
Gln29 predicted: No results obtained, sequencing might be blocked
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
39.3 kDa (monomer)
50-60 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SECTM1

SECTM1 (secreted and transmembrane 1), also called K12, is either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form (1, 2). SECTM1 protein expression is detected in some myeloid cells, such as stimulated monocytes, immature monocyte‑derived dendritic cells, and granulocytes (1‑3). In the thymus, it is also expressed by epithelia and fibroblasts (2). Stimulation with IFN‑ gamma is often necessary to detect SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Human SECTM1 cDNA encodes 248 amino acids (aa), including a 28 aa signal sequence, a 117 aa extracellular domain (ECD) with one potential N‑linked glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic sequence (1). The ECD shows some similarity to Ig domains (1). The human SECTM1 ECD shares limited (approximately 45‑51%) aa sequence identity with mouse SECTM1 (a or b) and rat SECTM1 ECD. SECTM1 from human and mouse each show species‑specific binding of CD7 and co‑stimulate CD4+ and CD8+ T cells through CD7, either alone or through synergy with CD28 (2‑4). Immobilized recombinant human SECTM1/Fc also enhances human NK cell expression of activation markers such as CD25, CD69 and CD54 (4).

  1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
  2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
  3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
  4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
  5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.
Long Name
Secreted and Transmembrane 1
Entrez Gene IDs
6398 (Human)
Alternate Names
CD7 Ligand; K12secreted and transmembrane protein 1; Protein K12; Protein K-12; secreted and transmembrane 1; SECTM1; type 1a transmembrane protein


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