Recombinant Human Semaphorin 3B Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9518-S3-025
Recombinant Human Semaphorin 3B Fc Chimera Protein Bioactivity
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Citations (1)
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Recombinant Human Semaphorin 3B Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Semaphorin 3B Fc Chimera is coated at 1 μg/mL, 100 μL/well, it binds to Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) with an ED50 of 25-150 ng/mL.
Source
Trichoplusia ni, T. ni (baculovirus)-derived human Semaphorin 3B protein
Human Semaphorin 3B
(Ala25-Gly727(Arg551Ala, Arg554Ala))
Accession # Q13214-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
105 kDa
SDS-PAGE
96-116 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9518-S3

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid, NaCl and Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Semaphorin 3B Fc Chimera Protein Bioactivity View Larger

When Recombinant Human Semaphorin 3B Fc Chimera (Catalog # 9618-S3) is coated at 1 µg/mL, 100 µL/well, it binds to Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) with an ED50 of 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3B

Semaphorin 3B (Sema3B), also known as Semaphorin V and Sema A(V), is an approximately 90 kDa member of the semaphorin family that plays a role in cancer, neuronal and vascular development (1-4). Class 3 semaphorins are secreted molecules found in vertebrates and contain a common domain structure. The Sema3B domains consist of a 484 aa Sema domain, followed by a plexin-semaphorin-integrin (PSI) domain, an 87 aa Ig-like C2-type domain, and a 21 aa basic domain (5). Within these domains, human Sema3B shares 88% and 90% aa sequence identity with mouse and rat Sema3B, respectively. Like other Class 3 semaphorins, Sema3B signaling is transduced by a transmembrane plexin-A dimer, which also has a Sema domain and is coupled to kinase pathways (6, 7). Sema3B binds indirectly to plexins, and requires interaction with neuropilins for activity (6, 7). Repulsive signals through Neuropilin-1 (Npn-1) and attractive signals through Neuropilin-2 (Npn-2) have been identified, affecting adhesion molecule activity in opposite directions (6, 8). In tumors, Sema3B competes with VEGF165 for binding to Npn-1, which in part down-regulates tumor angiogenesis, inhibits tumor cell proliferation and promotes apoptosis (9, 10). However, it is also proposed to promote dissemination and metastatic progression by stimulating Npn-1 mediated IL-8 secretion in a subset of tumors that over-express Sema3B (11). In differentiating osteoblasts, Sema3B expression is induced by vitamin D and promotes osteoclastogenesis (12).

References
  1. Sekido, Y. et al. (1996) Proc Natl Acad Sci U S A. 93:4120.
  2. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
  3. Sabag, A. et al. (2012) PLoS One. 7:e42912.
  4. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
  5. Yazdani, U. and J. Terman. (2006) Genome Biology. 7:211.
  6. Halloran, M. C. and M. A. Wolman (2006) Curr. Opin. Cell Biol. 18:533.
  7. Neufeld, G. and O. Kessler (2008) Nat. Rev. Cancer 8:632.
  8. Falk, J. et al. (2005) Neuron 48:63.
  9. Castro-Rivera, E. et al. (2004) Proc. Natl. Acad. Sci. USA 101:11432.
  10. Koyama, N. et al. (2008) Oncogene 27:6581.
  11. Rolny, C. et al. (2008) J. Exp. Med. 205:1155.
  12. Sutton, A. L. M. et al. (2008) Mol. Endocrinol. 22:1370.
Entrez Gene IDs
7869 (Human); 20347 (Mouse); 363142 (Rat)
Alternate Names
(semaphorin) 3B; LUCA-1; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema V; SemA; SEMA3B; SEMA5; SEMA5FLJ34863; SEMAA; Semaphorin 3B; semaphorin A; semaphorin V; semaphorin-3B; semaphorin-V; SEMAV

Citation for Recombinant Human Semaphorin 3B Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Class 3 semaphorins modulate the invasive capacity of rheumatoid arthritis fibroblast-like synoviocytes
    Authors: MW Tang, B Malvar Fer, SP Newsom, JD van Buul, TRDJ Radstake, DL Baeten, PP Tak, KA Reedquist, S García
    Rheumatology (Oxford), 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation

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