Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

Recombinant Human Semaphorin 3D Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Semaphorin 3D

Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2). Class 3 semaphorins are secreted molecules, and contain a common domain structure. The human Sema3D domains consist of a 488 amino acid (aa) Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4). Within these domains, human Sema3D shares 94% aa sequence identity with mouse and rat Sema3D. Sema3D induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (5, 6). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (7). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (7). Sema3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).

Related Research Areas

• Axon Guidance and Neuron Migration
• Proteoglycans
• Semaphorins, Plexin Receptors, and Related Molecules