Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9674-S3-025
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human Semaphorin 3D Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Semaphorin 3D Fc Chimera is coated at 1 µg/mL, 100 μL/well, Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) binds with an ED50 of 0.5-2.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3D protein
Human Semaphorin 3D
(Thr37-His736 (Arg571Ala) & (Arg572Ala))
Accession # O95025
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr37
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
107 kDa
SDS-PAGE
98-113 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9674-S3

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Semaphorin 3D

Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2). Class 3 semaphorins are secreted molecules, and contain a common domain structure. The human Sema3D domains consist of a 488 amino acid (aa) Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4). Within these domains, human Sema3D shares 94% aa sequence identity with mouse and rat Sema3D. Sema3D induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (5, 6). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (7). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (7). Sema3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).

References
  1. Sabag, A. et al. (2012) PLoS One. 7:e42912.
  2. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
  3. Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
  4. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
  5. Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
  6. Wolman, M.A. et al. (2007) J. Neurobiology. 27:9653.
  7. Aghajanian, H. et al. (2015) Nature Commun. 7:12038.
Entrez Gene IDs
223117 (Human); 108151 (Mouse); 246262 (Rat)
Alternate Names
(semaphorin) 3D; coll-2; Collapsin 2; MGC39708; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; SEMA3D; Semaphorin 3D; Semaphorin-3D; Sema-Z2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Semaphorin 3D Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human Semaphorin 3D Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review