Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Semaphorin 3D Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human Semaphorin 3D Fc Chimera is coated at 1 µg/mL, 100 μL/well, Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) binds with an ED50 of 0.5-2.5 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3D protein
Human Semaphorin 3D
(Thr37-His736 (Arg571Ala) & (Arg572Ala))
Accession # O95025
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
107 kDa
98-113 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Semaphorin 3D

Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2). Class 3 semaphorins are secreted molecules, and contain a common domain structure. The human Sema3D domains consist of a 488 amino acid (aa) Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4). Within these domains, human Sema3D shares 94% aa sequence identity with mouse and rat Sema3D. Sema3D induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (5, 6). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (7). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (7). Sema3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).

  1. Sabag, A. et al. (2012) PLoS One. 7:e42912.
  2. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
  3. Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
  4. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
  5. Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
  6. Wolman, M.A. et al. (2007) J. Neurobiology. 27:9653.
  7. Aghajanian, H. et al. (2015) Nature Commun. 7:12038.
Entrez Gene IDs
223117 (Human); 108151 (Mouse); 246262 (Rat)
Alternate Names
(semaphorin) 3D; coll-2; Collapsin 2; MGC39708; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; SEMA3D; Semaphorin 3D; Semaphorin-3D; Sema-Z2


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