Recombinant Human Semaphorin 4A Fc Chimera Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
|Human Semaphorin 4A
Accession # Q9H3S1
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Semaphorin 4A
Semaphorin 4A (Sema4A, previously semB) is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems (1). The 761 amino acid (aa) human Sema4A precursor contains a 32 aa signal sequence, a 651 aa extracellular domain (ECD) containing sema, PSI and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 57 aa cytoplasmic domain with two Ser/Thr phosphorylation sites (2). Human Sema4A ECD shares 87%, 87%, 86% and 85% aa identity with mouse, rat, bovine and canine Sema4A, respectively, and shares 32-37% aa identity with other human Sema4 family members. Of six reported splice variants with 723, 629, 370, 321, 236 and 220 aa, five lack the N-terminus and/or portions of the sema domain, and three lack the transmembrane and cytoplasmic domains in the C-terminus (3). Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2 (4). Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses (5). Roles for Sema4A have also been identified in the brain, the endothelium and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with plexin-B1 (6). Interaction of Sema4A with endothelial cell plexin-D1 causes opposition to the angiogenic, proliferative, chemotactic and integrin-mediated adhesive actions of VEGF (7). The retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans (8, 9).
- Kumanogoh, A. et al. (2003) J. Cell Sci. 116:3463.
- Swissprot Accession # Q9H3S1.
- Entrez Accession # CAI15528, CAI15529, CAI15531, CAI15532, CAI15533 and EAW52993.
- Kumanogoh, A. et al. (2002) Nature 419:629.
- Kumanogoh, A. et al. (2005) Immunity 22:305.
- Yukawa, K. et al. (2005) Int. J. Mol. Med. 16:115.
- Toyofuku, T. et al. (2007) EMBO J. 26:1373.
- Rice, D.S. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:2767.
- Abid, A. et al. (2007) J. Med. Genet. 43:378.
Citations for Recombinant Human Semaphorin 4A Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
Authors: Delgoffe, Greg M, Woo, Seng-Ryo, Turnis, Meghan E, Gravano, David M, Guy, Cliff, Overacre, Abigail, Bettini, Matthew, Vogel, Peter, Finkelstein, David, Bonnevier, Jody, Workman, Creg J, Vignali, Dario A
Sample Types: N/A
Applications: Binding Assay
Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
Authors: Meda C, Molla F, De Pizzol M
J. Immunol., 2012;188(8):4081-92.
Sample Types: Whole Cells
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