Recombinant Human Semaphorin 5B Fc Chimera Protein, CF

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Recombinant Human Semaphorin 5B Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. When 5 x 104 cells/well are added to Recombinant Human Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is 0.7‑2.8 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 5B protein
Human Semaphorin 5B
(Val78 - Thr834)
Accession # NP_001026872
(Pro100 - Lys330)
Accession #
N-terminal Sequence
Predicted Molecular Mass
110.3 kDa (monomer)
130-140 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 5B

Semaphorin 5B (Sema5B, previously called SemG) is a ~150 kDa protein of the semaphorin family of axon guidance molecules (1, 2). Class 5 semaphorins are 1-pass transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains (1, 2). Sema5B is expressed developmentally in the neuroepithelium along the anterior-posterior axis in a complimentary pattern with Sema5A (2, 3). It has been identified in oligodendrocytes and their precursors, neurons, dental mesenchyme and pre‑osteoblasts (1 ‑ 6). Sema5B expression may be up‑regulated in cancer cells such as renal carcinoma clear cells, where blocking Sema5B expression attenuates viability (7). The human Sema5B cDNA encodes a 1151 amino acid (aa) type III transmembrane protein with a 1036 aa extracellular domain (ECD), a 21 aa transmembrane sequence and a 94 aa cytoplasmic portion. Within aa 78 ‑ 834, which includes the sema domain and two of the five TSP repeats, human Sema5B shares 94 ‑ 95% aa identity with corresponding mouse, rat, equine and bovine sequences. An alternate start site at aa 59 and two splicing variations in the cytoplasmic domain may create isoforms of 1093, 1092 or 1130 aa, respectively (7, 8). In the hippocampus, either full-length or proteolytically created secreted Sema5B mediates neuronal synapse elimination (6). In the cortex, Sema5B appears to be inhibitory to cortical axons, but not to dorsal thalamic axons, indicating a role in corticothalamic axon guidance during development (3). Sema5B promotes the influx of extracellular calcium, which is necessary to induce neuronal growth cone collapse (9). Semaphorins typically transduce signals through transmembrane plexins, but additional molecules, such as heparin sulfate or chondroitin sulfate proteoglycans which bind TSP repeats on the class 5 family member Sema5A, can mediate midbrain axon attraction or repulsion (10).

  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Adams, R.H. et al. (1996) Mech. Dev. 57:33.
  3. Lett, R.L.M. et al. (2009) Cerebral Cortex 19:1408.
  4. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  5. Cohen, R.I. et al. (2003) J. Neurochem. 85:1262.
  6. O’Connor, T.P. et al. (2009) Neural Dev. 4:18.
  7. Hirota, E. et al. (2006) Int. J. Oncol. 29:799.
  8. Swiss-Prot Accession # Q9P283
  9. To, K.C.W. et al. (2007) Mol. Cell. Neurosci. 36:425.
  10. Kantor, D.B. et al. (2004) Neuron 44:961.
Entrez Gene IDs
54437 (Human); 20357 (Mouse)
Alternate Names
FLJ10372; KIAA1445; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B; SEMA5B; SEMAG; Semaphorin 5B; SEMG

Citation for Recombinant Human Semaphorin 5B Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement During Development
    Authors: JS Jung, KD Zhang, Z Wang, M McMurray, A Tkaczuk, Y Ogawa, R Hertzano, TM Coate
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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