Recombinant Human Serpin A6 Protein, CF

Catalog # Availability Size / Price Qty
9580-PI-050
Recombinant Human Serpin A6 Protein Bioactivity
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Recombinant Human Serpin A6 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Cortisol-BSA Conjugate is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Serpin A6 binds with an ED50 of 1.5-7.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Serpin A6 protein
Met23-Val405, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met23
Predicted Molecular Mass
43 kDa
SDS-PAGE
50-70 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9580-PI

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Trehalose.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Serpin A6 Protein Bioactivity View Larger

When Cortisol-BSA Conjugate is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Serpin A6 (Catalog # 9580-PI) binds with an ED50 of 1.5-7.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A6

Corticosteroid-binding globulin (CBG), also known as Serpin A6, is an approximately 42 kDa member of the serpin superfamily of serine protease inhibitors. CBG belongs to the clade A subgroup of serpins which include inflammatory response molecules (1). Human CBG shares 57% sequence identity to mouse CBG. CBG is one of two serpins known to bind and transport hormones. CBG is responsible for binding ~90% of cortisol in a reversible manner to regulate levels under normal physiological states (2). However, cleavage of CBG's reactive site loop by proteases present during an inflammatory response leads to a targeted and immediate release of the anti-inflammatory steroid, cortisol, from CBG (3). Reduced cleavage of CBG is implicated in the inflammatory phenotype seen in obesity, metabolic syndrome, and rheumatoid arthritis (4, 5). Cleavage of CBG can be modulated by glycosylation (6). Naturally occurring mutations of CBG lead to abnormal levels or function (7, 8). Loss of functional CBG results in low cortisol levels in patients experiencing chronic pain, fatigue, hypotension and excess weight (8).

References
  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Lewis, J. G. et al (2005) Clin. Chem. Acta. 359:189.
  3. Klieber M.A. et al. (2007) J. Biol. Chem. 282:29594.
  4. Nenke, M.A. et al. (2016) Horm. Metab. Res. 48:523.
  5. Nenke, M.A. et al. (2016) Clin. Endocrinol. 85:369.
  6. Sumer-Bayraktar, Z. et al. (2016) J. Biol. Chem 291:17727.
  7. Simard, M. et al. (2015) J. Clin. Endocrinol. Metab. 100:E129.
  8. Gagliardi, L. et al. (2010) Mol. Cell. Endocrinol. 316:24.
Entrez Gene IDs
866 (Human); 12401 (Mouse); 299270 (Rat)
Alternate Names
antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin

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