Recombinant Human SEZ6L Protein, CF

R&D Systems | Catalog # 5598-S6

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human SEZ6L Protein (5598-S6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9BYH1

Applications

Bioactivity
View all SEZ6L Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SEZ6L protein
Leu29-Ala958, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

102 kDa

SDS-PAGE

130-200 kDa, reducing conditions

Activity

Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro‑2A mouse neuroblastoma cells.
1 μg/mL of Recombinant Human SEZ6L is able to signifcantly induce cell clumping and inhibit neurite outgrowth.

Formulation, Preparation, and Storage

5598-S6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEZ6L

Seizure 6-like Protein (SEZ6L) is a member of the SEZ6 family of proteins. It has a predicted molecular weight of approximately 110 kDa; however, human SEZ6L contains 11 potential N-linked glycosylation sites, and native mouse BSRP/SEZ6L is approximately 200 kDa (1, 2). Alternative splicing of the human Sez6l gene may generate at least ten isoforms (2). Human SEZ6L is a type I transmembrane glycoprotein composed of an extracellular domain (ECD) that contains two CUB domains and five short consensus repeats (SCRs) (2). It shares 80% and 81% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. SEZ6L is expressed in the plasma membrane and endoplasmic reticulum of neurons and lung bronchiolar epithelium and likely regulates PKC alpha activation (1, 2). Human SEZ6L has been shown to be involved in the maintenance of cellular cholesterol homeostasis (3). Additionally, in mouse pancreatic beta  cells, SEZ6L has been shown to be a substrate for BACE-2, which cleaves its ECD at a site 16 aa upstream from its transmembrane domain (4). BSRP-C, also known as SEZ6, has been reported to inhibit neurite outgrowth in mice (5). R&D Systems in-house testing shows that human SEZ6L also exhibits this function. Genetic variations in human Sez6l have been associated with bipolar disorder I and an increased risk for lung cancer (2, 6, 7).

References

  1. Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.
  2. Nishioka, M. et al. (2000) Oncogene 19:6251.
  3. Blattmann, P. et al. (2013) PLoS Genetics 9:e1003338.
  4. Stützer, I. et al. (2013) J. Biol. Chem. 288:10536.
  5. Gunnersen, J.M. et al. (2007) Neuron 56:621.
  6. Gorlov, I.P. et al. (2007) Cancer Res. 67:8406.
  7. Xu, C. et al. (2013) J. Affect. Disord. 145:95.

Long Name

Seizure-related 6 Homolog-like

Alternate Names

Acig1, AIG1, Aig1L, BSRP-B, SEZ6L

Entrez Gene IDs

23544 (Human); 56747 (Mouse); 304554 (Rat)

Gene Symbol

SEZ6L

UniProt

Q9BYH1 (Human)

Additional SEZ6L Products

Product Documents for Recombinant Human SEZ6L Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SEZ6L Protein, CF

For research use only

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