Recombinant Human sFRP-1 (Histidine-tagged) Protein

Carrier Free

Catalog # Availability Size / Price Qty
1384-SF-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1384-SF-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (8)
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Recombinant Human sFRP-1 (Histidine-tagged) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED50 for this effect is 2.5-10 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human sFRP-1 protein
Ser32-Lys314 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser32 & Phe39 & Asp42
Predicted Molecular Mass

33 kDa

SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

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1384-SF (with carrier)

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1384-SF/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1384-SF

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1384-SF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: sFRP-1

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-1 shares 97% and 96% amino acid sequence identity with mouse and rat sFRP-1, respectively. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is upregulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).

References
  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Finch, P.W. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.
Long Name
Secreted Frizzled Related Protein 1
Entrez Gene IDs
6422 (Human); 20377 (Mouse); 84402 (Rat)
Alternate Names
FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1

Citations for Recombinant Human sFRP-1 (Histidine-tagged) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. WIF1 prevents Wnt5A mediated LIMK/CFL phosphorylation and adherens junction disruption in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    J Inflamm (Lond), 2017-05-19;14(0):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    Cell Adh Migr, 2016-05-09;11(1):24-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Wnt activation by wild type and mutant myocilin in cultured human trabecular meshwork cells.
    Authors: Shen X, Ying H, Yue B
    PLoS ONE, 2012-09-13;7(9):e44902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009-05-28;62(4):510-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
    Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
    Exp. Hematol., 2008-06-17;36(9):1121-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women.
    Authors: Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M
    Arthritis Rheum., 2007-10-01;56(10):3319-25.
    Applications: ELISA (Standard)
  7. Wnt3a binds to several sFRPs in the nanomolar range.
    Authors: Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L
    Biochem. Biophys. Res. Commun., 2007-04-19;357(4):1119-23.
    Species: Mouse
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay, Surface Plasmon Resonance
  8. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006-08-30;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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