Recombinant Human SHBG Protein, CF

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Recombinant Human SHBG Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Bioassay data are not available.
Mouse myeloma cell line, NS0-derived human SHBG protein
Leu30-His402, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
41 kDa
41-52 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: SHBG

Human SHBG (Sex hormone-binding globulin), also known as ABP (Testis-specific Androgen-binding protein) or TeBG (Testosterone-estradiol-binding globulin), is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family (1). Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G‑ like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G‑ like domain (2). SHBG is synthesized by the liver and circulates in blood. It binds to certain androgens and estrogens and serves as a transporter for the bound steroids (3); it is also involved in receptor mediated processes (4). Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG. Low SHGB levels are associated with increased risk for type 2 diabetes for both man and women (5).

  1. Awakumov G.V. et al. (2010) Mol. Cell Endocrinol. 5:316.
  2. Grishkovskaya I. et al. (2000) EMBO J. 19:504.
  3. Anderson D.C. (1974) Clin. Endocrinol. (Oxf.) 3:69.
  4. Rosner W. et al. (2010) Mol. Cell Endocrinol.5:795.
  5. Muka T. et al. (2017) Diabetes. 66:577.
Long Name
Sex Hormone-binding Globulin
Entrez Gene IDs
6462 (Human)
Alternate Names
ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin


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