Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2859-SL-050
Product Details
Citations (2)
FAQs
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Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated Neu5Ac alpha 2-6GalNAc alpha (sialyl-Tn). Patel, N. et al. (1999) The Journal of Biological Chemistry 274:22729.
Source
Chinese Hamster Ovary cell line, CHO-derived human Siglec-6/CD327 protein
Human Siglec-6
(Gln27-Val331)
Accession # NP_942142
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln27 predicted
Predicted Molecular Mass
60.3 kDa (monomer)
SDS-PAGE
80-95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2859-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Siglec-6/CD327

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1 - 4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6 - 9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

References
  1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
  5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
  6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
  7. GenBank Accession # NP_942143.
  8. GenBank Accession #: NP_942142.
  9. GenBank Accession #: NP_001236.
  10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Long Name
Sialic Acid Binding Ig-like Lectin 6
Entrez Gene IDs
946 (Human)
Alternate Names
CD327 antigen; CD327; CD33 antigen-like 1; CD33L1; CD33L1OBBP1CD327; CD33L2; CD33LCDW327; CDw327; OB-BP1; Obesity-binding protein 1; sialic acid binding Ig-like lectin 6; sialic acid-binding Ig-like lectin 6; Siglec6; Siglec-6

Citations for Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
    Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
    Elife, 2014;3(0):e04066.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway.
    Authors: Lam KK, Chiu PC, Lee CL
    J. Biol. Chem., 2011;286(43):37118-27.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay

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