Recombinant Human Siglec-8 Fc Chimera Protein, CF
Recombinant Human Siglec-8 Fc Chimera Protein, CF Summary
Accession # AAH53319
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs (2, 3). Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs (4, 5). Siglec-8 is expressed on eosinophils, basophils, and mast cells (2, 3), and it shows a binding preference for the carbohydrate 6-O sulfated sLex (3, 6, 7). In mouse, Siglec-F is a functional paralog of human Siglec-8, as shown by its similar expression profile and ligand specificity (8). Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF (10-12).
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- Foussias, G. et al. (2000) Biochem. Biophys. Res. Commun. 278:775.
- Bochner, B.S. et al. (2005) J. Biol. Chem. 280:4307.
- Hudson, S.A. et al. (2011) J. Clin. Immunol. 31:1045.
- Tateno, H. et al. (2005) Glycobiology 15:1125.
- Yokoi, H. et al. (2008) J. Allergy Clin. Immunol. 121:499.
- Kano, G. et al. (2013) J. Allergy Clin. Immunol. 132:437.
- Nutku, E. et al. (2003) Blood 101:5014.
- Na, H.J. et al. (2012) Cytokine 57:169.
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