Recombinant Human Siglec-8 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9045-SL-050
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Recombinant Human Siglec-8 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Biotinylated Sialyl Lewis X was immobilized at 0.05 μg/mL (100 μL/well), it binds Recombinant Human Siglec-8 Fc Chimera. The concentration of Human Siglec-8 Fc Chimera that produces 50% of the optimal binding response is approximately 0.2-1.2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Siglec-8 protein
Human Siglec-8
(Met17-Ala363)
Accession # AAH53319
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met17
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
64 kDa
SDS-PAGE
68-88 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9045-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-8

Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs (2, 3). Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs (4, 5). Siglec-8 is expressed on eosinophils, basophils, and mast cells (2, 3), and it shows a binding preference for the carbohydrate 6-O sulfated sLex (3, 6, 7). In mouse, Siglec-F is a functional paralog of human Siglec-8, as shown by its similar expression profile and ligand specificity (8). Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF (10-12).

References
  1. Kiwamoto, T. et al. (2013) Curr. Opin. Allergy Clin. Immunol. 13:106.
  2. Kikly, K.K. et al. (2000) J. Allergy Clin. Immunol. 105:1093.
  3. Floyd, H. et al. (2000) J. Biol. Chem. 275:861.
  4. Aizawa, H. et al. (2002) J. Allergy Clin. Immunol. 109:176.
  5. Foussias, G. et al. (2000) Biochem. Biophys. Res. Commun. 278:775.
  6. Bochner, B.S. et al. (2005) J. Biol. Chem. 280:4307.
  7. Hudson, S.A. et al. (2011) J. Clin. Immunol. 31:1045.
  8. Tateno, H. et al. (2005) Glycobiology 15:1125.
  9. Yokoi, H. et al. (2008) J. Allergy Clin. Immunol. 121:499.
  10. Kano, G. et al. (2013) J. Allergy Clin. Immunol. 132:437.
  11. Nutku, E. et al. (2003) Blood 101:5014.
  12. Na, H.J. et al. (2012) Cytokine 57:169.
Long Name
Sialic Acid Binding Ig-like Lectin 8
Entrez Gene IDs
27181 (Human)
Alternate Names
CD329 antigen; CDw329; MGC59785; SAF2; SAF2SAF-2; sialic acid binding Ig-like lectin 8; Sialoadhesin family member 2; Siglec8; Siglec-8; SIGLEC8L; SIGLEC8Lsialic acid-binding Ig-like lectin 8

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