Recombinant Human Siglec-9 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1139-SL-050
Product Details
Citations (9)
FAQs
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Recombinant Human Siglec-9 Fc Chimera Protein, CF Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is 1‑5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Siglec-9 protein
Human Siglec-9
(Gln18-Gly348)
Accession # Q9Y336
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln18 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
62.7 kDa (monomer)
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1139-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

References
  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
  6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.
Long Name
Sialic Acid Binding Ig-like Lectin 9
Entrez Gene IDs
27180 (Human)
Alternate Names
CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; Siglec9; Siglec-9

Citations for Recombinant Human Siglec-9 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
    Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
    PLoS ONE, 2019;14(7):e0219480.
    Species: Human
    Sample Types: Natural Protein
    Applications: Bioassay
  2. Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment
    Authors: Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten
    Cancer Immunol Res, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Siglec-9 is upregulated in rheumatoid arthritis and suppresses collagen-induced arthritis through reciprocal regulation of Th17/Treg cell differentiation
    Authors: X Wang, D Liu, Y Ning, J Liu, X Wang, R Tu, H Shen, Q Chen, Y Xiong
    Scand. J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Increased expression of Siglec-9 in chronic obstructive pulmonary disease
    Authors: Z Zeng, M Li, M Wang, X Wu, Q Li, Q Ning, J Zhao, Y Xu, J Xie
    Sci Rep, 2017;7(1):10116.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity
    Authors: T Ito, M Ishigami, Y Matsushita, M Hirata, K Matsubara, T Ishikawa, H Hibi, M Ueda, Y Hirooka, H Goto, A Yamamoto
    Sci Rep, 2017;7(0):44043.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. Soluble Siglec-9 suppresses arthritis in a collagen-induced arthritis mouse model and inhibits M1 activation of RAW264.7 macrophages
    Authors: Takuya Matsumoto
    Arthritis Res Ther, 2016;18(1):133.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  7. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
    Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
    J Neurosci, 2015;35(6):2452-64.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
    Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
    Elife, 2014;3(0):e04066.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  9. Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
    Authors: Zou Z, Chastain A, Moir S
    PLoS ONE, 2011;6(9):e24559.
    Species: Virus
    Sample Types: Virus
    Applications: Surface Plasmon Resonance

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