Recombinant Human SIRP beta 1/CD172b Protein, CF

Catalog # Availability Size / Price Qty
9978-SB-050
Recombinant Human SIRP beta 1/CD172b Protein, CF Binding Activity
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Recombinant Human SIRP beta 1/CD172b Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP beta 1/CD172b (Catalog # 9978-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED50 of 0.1-0.8 μg/mL
Source
Human embryonic kidney cell, HEK293-derived human SIRP beta 1/CD172b protein
Human SIRP beta 1/CD172b
(Glu30-Ala369)
Accession # O00241
HH
Hemagglutinin Tag
(YPYDVPDYA)
N-terminusC-terminus
N-terminal Sequence
Analysis
Glu30
Structure / Form
Dimer, non covalent
Predicted Molecular Mass
38 kDa
SDS-PAGE
45-61 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9978-SB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human SIRP beta 1/CD172b (Catalog # 9978-SB) isimmobilized at 1 µg/mL, Recombinant Human SP‑D (Catalog # 1920-SP) binds with anED50 of 0.1-0.8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human SIRP beta 1/CD172b (Catalog #9978-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by Coomassie® Blue staining, showing bands at 45-61 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP beta 1/CD172b

Signal-regulatory protein beta 1 (SIRP beta 1) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. The extracellular domain contains 3 intramolecular disulfide bonds and one interchain disulfide at Cys320. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Human SIRP beta 1 shares a 57% sequence identity with mouse and rat SIRP beta 1. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5,6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma  receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

References
  1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
  3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132
  4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
  5. Dietrich J. et al. (2000) J Immunol. 164:9.
  6. Brook G. et al. (2004) J Immunol. 173:2562.
  7. Hayashi A. et al. (2004) J Biol Chem. 279:29450.
  8. Fournier B. et al. (2012) J. Biol. Chem. 287:19386.
  9. Seiffert M. et al. (2001) Blood 97:2741.
Long Name
Signal-regulatory Protein beta 1
Entrez Gene IDs
10326 (Human); 102139733 (Cynomolgus Monkey)
Alternate Names
CD172 antigen-like family member B; CD172b antigen; CD172b; DKFZp686A05192; FLJ26614; signal-regulatory protein beta 1; signal-regulatory protein beta-1; SIRP beta 1; SIRPB1; SIRP-beta-1 isoform 3; SIRP-BETA-1

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