Recombinant Human SIRP delta Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human SIRP delta Fc Chimera Protein, CF Binding Activity
2 Images
Product Details

Recombinant Human SIRP delta Fc Chimera Protein, CF Summary

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) is immobilized at 1 µg/mL (100 µL/well), recombinant human DIRAS2 binds with an ED50 of 0.15-0.9 μg/mL.
Human embryonic kidney cell, HEK293-derived human SIRP delta protein
Human SIRP delta
Accession # Q9H106
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa
50-60 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human SIRP delta Fc Chimera (Catalog #10138-SB) is immobilized at 1 µg/mL, recombinant human DIRAS2 binds with anED50 of 0.15-0.9 μg/mL.


2 μg/lane of Recombinant Human SIRP delta Fc Chimera (Catalog# 10138-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by Coomassie® Blue staining, showing bands at 50-60 kDaand 100-120 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: SIRP delta

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

  1. Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  2. Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
  3. Huttlin, E.L. et al. (2017) Nature 545:505.
Long Name
Signal-regulatory Protein delta
Entrez Gene IDs
128646 (Human)
Alternate Names
dJ576H24.4; Protein tyrosine phosphatase non-receptor type substrate 1-like 2; protein tyrosine phosphatase, non-receptor type substrate 1-like 2; PTPNS1L2; signal-regulatory protein delta; SIRP delta; SIRPD; SIRP-Delta


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human SIRP delta Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human SIRP delta Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human SIRP delta Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review