Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
4486-SBB-100
Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF Bioactivity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8-4.8 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human SIRP gamma/CD172g protein
Human SIRP gamma
(Glu29-Pro360)
Accession # Q9P1W8-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
63 kDa
SDS-PAGE
70-80 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4486-SBB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile PBS
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human SIRP gamma /CD172g Fc Chimera inhibitsanti-CD3-induced proliferation of stimulated human T cells. The ED50 for thiseffect is 0.8-4.8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human SIRP gamma was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 70-80 kDa and 140-160 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SIRP gamma/CD172g

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are "paired receptors" with homology in the extracellular domain but variability in the C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling motif (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5). Engagement of CD47 by SIRP gamma was shown to induce apoptosis on T-cell and monocyte cell lines (6).

References
  1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
  2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
  5. Piccio, L. et al. (2005) Blood 105:2421.
  6. Brooke, G. et al. (2004) J. Immunol. 173:2562.
Long Name
Signal-regulatory Protein gamma
Entrez Gene IDs
55423 (Human); 102138723 (Cynomolgus Monkey)
Alternate Names
bA77C3.1; CD172g antigen; CD172g; FLJ42230; signal-regulatory protein beta 2; Signal-regulatory protein beta-2; signal-regulatory protein gamma; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPB2CD172 antigen-like family member B; SIRP-beta-2; SIRPG; SIRPgamma; SIRP-gamma

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review