Recombinant Human SIRP gamma/CD172g His-tag Protein, CF

R&D Systems | Catalog # 9999-SB

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human SIRP gamma/CD172g His-tag Protein (9999-SB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q9P1W8

Applications

Bioactivity
View all SIRP gamma/CD172g Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human SIRP gamma/CD172g protein
Glu29-Pro360, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu29

Predicted Molecular Mass

38 kDa

SDS-PAGE

38-48 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human SIRP gamma /CD172g binds with an ED50 of 0.6-3.6 μg/mL.

Scientific Data Images for Recombinant Human SIRP gamma/CD172g His-tag Protein, CF

Recombinant Human SIRP gamma/CD172g His-tag Protein Binding Activity

Recombinant Human SIRP gamma/CD172g His-tag Protein Binding Activity

When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) is immobilized at 0.5 µg/mL, Recombinant Human SIRP gamma/CD172g (Catalog # 9999-SB) binds with an ED50 of 0.6-3.6 µg/mL.

Recombinant Human SIRP gamma/CD172g His-tag Protein SDS-PAGE

Recombinant Human SIRP gamma/CD172g His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human SIRP gamma /CD172g was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-48 kDa.

Formulation, Preparation, and Storage

9999-SB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP gamma/CD172g

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are "paired receptors" with homology in the extracellular domain but variability in the C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma  sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56 bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling mechanism (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and co-stimulate T cell activation (5). Engagement of CD47 by SIRP gamma was shown to induce apoptosis on T-cell and monocyte cell lines (6).

References

  1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
  2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
  5. Piccio, L. et al. (2005) Blood 105:2421.
  6. Brooke, G. et al. (2004) J. Immunol. 173:2562.

Long Name

Signal-regulatory Protein gamma

Alternate Names

CD172g, SIRP beta 2, SIRPG

Entrez Gene IDs

55423 (Human); 102138723 (Cynomolgus Monkey)

Gene Symbol

SIRPG

UniProt

Q9P1W8

Additional SIRP gamma/CD172g Products

Product Documents for Recombinant Human SIRP gamma/CD172g His-tag Protein, CF

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Product Specific Notices for Recombinant Human SIRP gamma/CD172g His-tag Protein, CF

For research use only

Related Research Areas

T Cell Migration/Adhesion

Citations for Recombinant Human SIRP gamma/CD172g His-tag Protein, CF

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