Recombinant Human SLITRK1 Protein, CF

R&D Systems | Catalog # 3009-SK

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human SLITRK1 Protein (3009-SK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q96PX8

Applications

Bioactivity
View all SLITRK1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SLITRK1 protein
Ala16-Ser616, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala16

Predicted Molecular Mass

68 kDa

SDS-PAGE

100-115 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.

Formulation, Preparation, and Storage

3009-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLITRK1

SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family includes six members, all of which contain a slit-like extracellular region, and all of which (save for SLITRK1) contain a Trk-like cytoplasmic region (3). Human SLITRK1 is a type I transmembrane protein 696 amino acids (aa) in length. It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 57 - 200; aa 374 - 517) contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR (aa 212 - 262; aa 529 - 579). The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD. Their significance is unclear (5). One truncating frameshift mutation is known that shows a unique 27 aa substitution after Arg 421 (6). This 448 aa form is associated with Tourette’s Syndrome. As noted, the cytoplasmic region is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2 - 6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is a protein that is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2 - 6 block neurite extension (3). Human SLITRK1 shares 99%, 98% and 99% aa sequence identity with canine, mouse and bovine SLITRK1, respectively.

References

  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Nagase, T. et al. (2001) DNA Res. 8:179.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Enkhbayar, P. et al. (2003) Proteins 54:394.
  5. Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.
  6. Abelson, J.F. et al. (2005) Science 310:317.

Long Name

SLIT and NTRK-like Family, Member 1

Alternate Names

LRRC12

Entrez Gene IDs

114798 (Human); 76965 (Mouse)

Gene Symbol

SLITRK1

UniProt

Q96PX8

Additional SLITRK1 Products

Product Documents for Recombinant Human SLITRK1 Protein, CF

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Product Specific Notices for Recombinant Human SLITRK1 Protein, CF

For research use only

Citations for Recombinant Human SLITRK1 Protein, CF

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