Recombinant Human SLURP2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10035-SP-050
Recombinant Human SLURP2 Fc Chimera Protein, CF Binding Activity
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Recombinant Human SLURP2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Nicotinic Acetylcholine R alpha 7/CHRNA7 (Novus Catalog # H00001139) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SLURP2 Fc Chimera binds with an ED50 of 1-6 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human SLURP2 protein
MDHuman IgG1
(Pro100-Lys330)
IEGRHuman SLURP2
(Ile23-Asp97)
Accession # P0DP57
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
35 kDa
SDS-PAGE
36-43 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10035-SP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant HumanNicotinic Acetylcholine R alpha 7/CHRNA7 (Novus Catalog # H00001139) is immobilized at 1 µg/mL (100 µL/well), RecombinantHuman SLURP2 Fc Chimera (Catalog # 10035-SP) binds with an ED50 of 1‑6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human SLURP2 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 36-43 kDa and 70-85 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLURP2

Secreted Ly-6/uPAR domain-containing protein 2 (SLURP2) is expressed primarily in epithelial and immune cells and regulates growth and differentiation of epithelial cells (1, 2). It is part of the Ly6/neurotoxin superfamily and contains the ‘three-finger' UPAR/Ly6 domain, which has a beta -structural core and three protruding loops (2, 3). Mature SLURP2 is a secreted protein which is comprised of 75 amino acids. Human SLURP2 shares 65% and 25% aa identity with mouse and rat SLURP2, respectively. SLURP2 is demonstrated to lessen the tumorigenic activity of nitrosamines on Het-1A cells (4), reduce inflammation on human intestinal epithelial cells and Immunocytes such as CEM and U937 (5). SLURP2 can interact with alpha 3, alpha 4, alpha 5, alpha 7, beta 2, beta 4, and possibly alpha 6 nicotinic acetylcholine receptors (nAChRs) as well as M1 and M3 muscarinic acetylcholine receptors (3, 6). In addition, SLURP2 controls the proliferation of keratinocytes and epithelial cancer cells via nAChRs on the cell surface (1, 6, 7). It has been suggested SLURP2 may be involved in the pathophysiology of psoriasis through its role in keratinocyte hyper-proliferation and/or T cell differentiation/activation (8).

References
  1. Arredondo, J. et al. (2006) J. Cell. Physiol. 208:238.
  2. Moriwaki, Y. et al. (2015) Int. Immunopharmacol. 29:71.
  3. Lyukmanova, E.N. et al. (2016) Sci. Rep. 6:30698.
  4. Arredondo, J. et al. (2007) Life. Sci. 80:2243.
  5. Chernyavsky, AI. et al. (2014) Biomed. Res. Int. 2014:609086.
  6. Lyukmanova, E.N. et al. (2014) Acta. Naturae. 6:60.
  7. Lyukmanova, E.N. et al. (2018) Br. J. Pharmacol. 175:1973.
  8. Tsuji, H. et al. (2003) Genomics 81:26.
Long Name
Secreted Ly-6/uPAR-related Protein 2
Entrez Gene IDs
432355 (Human)
Alternate Names
SLURP2; SLURP-2

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