Recombinant Human SLURP2 Fc Chimera Protein, CF Summary
Accession # P0DP57
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Nicotinic Acetylcholine R alpha 7/CHRNA7 (Novus Catalog # H00001139) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SLURP2 Fc Chimera (Catalog # 10035-SP) binds with an ED50of 1-6 μg/mL.
2 μg/lane of Recombinant Human SLURP2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-43 kDa and 70-85 kDa, respectively.
Secreted Ly-6/uPAR domain-containing protein 2 (SLURP2) is expressed primarily in epithelial and immune cells and regulates growth and differentiation of epithelial cells (1, 2). It is part of the Ly6/neurotoxin superfamily and contains the ‘three-finger' UPAR/Ly6 domain, which has a beta -structural core and three protruding loops (2, 3). Mature SLURP2 is a secreted protein which is comprised of 75 amino acids. Human SLURP2 shares 65% and 25% aa identity with mouse and rat SLURP2, respectively. SLURP2 is demonstrated to lessen the tumorigenic activity of nitrosamines on Het-1A cells (4), reduce inflammation on human intestinal epithelial cells and Immunocytes such as CEM and U937 (5). SLURP2 can interact with alpha 3, alpha 4, alpha 5, alpha 7, beta 2, beta 4, and possibly alpha 6 nicotinic acetylcholine receptors (nAChRs) as well as M1 and M3 muscarinic acetylcholine receptors (3, 6). In addition, SLURP2 controls the proliferation of keratinocytes and epithelial cancer cells via nAChRs on the cell surface (1, 6, 7). It has been suggested SLURP2 may be involved in the pathophysiology of psoriasis through its role in keratinocyte hyper-proliferation and/or T cell differentiation/activation (8).
- Arredondo, J. et al. (2006) J. Cell. Physiol. 208:238.
- Moriwaki, Y. et al. (2015) Int. Immunopharmacol. 29:71.
- Lyukmanova, E.N. et al. (2016) Sci. Rep. 6:30698.
- Arredondo, J. et al. (2007) Life. Sci. 80:2243.
- Chernyavsky, AI. et al. (2014) Biomed. Res. Int. 2014:609086.
- Lyukmanova, E.N. et al. (2014) Acta. Naturae. 6:60.
- Lyukmanova, E.N. et al. (2018) Br. J. Pharmacol. 175:1973.
- Tsuji, H. et al. (2003) Genomics 81:26.
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