Recombinant Human SorCS3 Protein, CF Summary
Ala134-Ser1125, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SorCS3 (sortilin-related YPS10 domain containing receptor 3) is a type I transmembrane receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family of sorting receptors. This family includes sortilin, SorLA, and three SorCS proteins (1, 2). It is synthesized as a 1222 amino acid (aa) preproform with a 33 aa signal sequence and a 100 aa propeptide. After proteolytic processing at a furin-type consensus sequence, mature SorCS3 is generated that is 100 - 110 kDa in size and 1089 aa in length. It contains a 992 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane segment and a 76 aa cytoplasmic domain. Human SorCS3 ECD shares 92%, 93%, 94% and 91% aa identity with mouse, rat, bovine and canine SorCS3 ECD, respectively. It also shares 70% and 46% aa identity with human SorCS1 and SorCS2 ECD, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain that binds both pro- and mature NGF (2, 3). The ECD may be shed constitutively, or upregulated when induced by phorbol esters (4). The metalloproteinase TACE/ADAM17 is known to cleave SorCS3 near the membrane (4). The shed ECD is able to bind PDGF-BB and the NGF propeptide (4, 5). The function of the SorCS3 propeptide is unknown. It does not block activity or influence protein processing in a manner analogous to the sortilin propeptide (3, 5). SorCS3 is predominantly expressed on the plasma membrane where it can be slowly internalized (3). Despite the presence of a sorting domain, there is no evidence for SorCS3-mediated intracellular trafficking activity (3). It is expressed in mouse embryonic and adult central nervous system in areas distinct from that of SorCS1 and SorCS2 (1). Neuronal activity upregulates SorCS3 expression in the mouse hippocampus (1).
- Hermey, G. et al. (2004) J. Neurochem. 88:1470.
- Hampe, W. et al. (2001) Hum. Genet. 108:529.
- Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.
- Hermey, G. et al. (2006) Biochem. J. 395:285.
- Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
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