Recombinant Human Sortilin Protein, CF

Catalog # Availability Size / Price Qty
3154-ST-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Sortilin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhSortilin at 4 µg/mL (100 µL/well) can bind rh beta -NGF with a linear range of 3-200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Sortilin protein
Ser78-Asn755, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser78
Structure / Form
Monomer
Predicted Molecular Mass
76.6 kDa
SDS-PAGE
95-100 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3154-ST

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Sortilin

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Human preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre- and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10 CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature human Sortilin shares 91% aa identity with mouse and rat Sortilin and 93% aa identity with dog. During murine development, sortilin is mainly expressed in the nervous system (5) where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (6 - 9). ProNGF (or the NGF propeptide alone) binds sortilin with much higher affinity (Kd ~5 - 8 nM) than does mature NGF (Kd ~90 nM). The complex of sortilin, pro-NGF and the receptor p75ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with proBDNF and BDNF (8, 9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

References
  1. Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599.
  2. Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221.
  3. Petersen, C.M. et al. (1998) EMBO J. 18:595.
  4. Nielsen, M.S. et al. (2001) EMBO J. 20:2180.
  5. Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216.
  6. Mazella, J. et al. (1998) J. Biol. Chem. 273:26273.
  7. Nykjaer, A et al. (2004) Nature 427:843.
  8. Teng, H.K. et al. (2005) J. Neurosci. 25:5455.
  9. Chen, Z-Y et al. (2004) J. Neurosci. 25:6156.
  10. Shi, J and K.V. Kandror (2005) Dev. Cell 9:99.
  11. Nielsen, M. S. et al. (1999) J. Biol. Chem. 274:8832.
Entrez Gene IDs
6272 (Human); 20661 (Mouse)
Alternate Names
100 kDa NT receptor; Glycoprotein 95; Gp95; Gp95LDLCQ6; Neurotensin receptor 3; NT3NTR3; Ntr3; SORT1; sortilin 1; Sortilin

Citation for Recombinant Human Sortilin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival.
    Authors: Fauchais AL, Lalloue F, Lise MC, Boumediene A, Preud'homme JL, Vidal E, Jauberteau MO
    J. Immunol., 2008;181(5):3027-38.
    Species: N/A
    Sample Types: N/A
    Applications: Antibody Absorption

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Recombinant Human Sortilin Protein, CF
By Anonymous on 10/13/2021
Application: Immunoassay Standard
Reason for Rating: Works great for ELISA assay