Recombinant Human Sphingosine Kinase 2/SPHK2 Protein, CF

Catalog # Availability Size / Price Qty
5298-SK-010
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Sphingosine Kinase 2/SPHK2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Sphingosine Kinase 2/SPHK2 protein
Asn2-Pro654, with an N-terminal 5-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
70 kDa
SDS-PAGE
70-85 kDa, reducing conditions

Product Datasheets

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5298-SK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5298-SK

Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: Sphingosine Kinase 2/SPHK2

Sphingosine kinases catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate, a lipid messenger molecule involved in the regulation of processes such as growth, differentiation, and cellular migration (1). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N-terminal and central regions (2). The two proteins also differ in tissue distribution and some kinetic properties (2). Sphingosine kinases can be either cytosolic or membrane-associated. Differences in the distribution of SPHK1 and SPHK2 within cells may be the cause of differences that have been observed in the function of the two enzymes, particularly their roles in apoptosis (3).

References
  1. Spiegel, S. (1999) J. Leukocyte Biol. 65:341.
  2. Liu, H. et al. (2000) J. Biol. Chem. 275:19513.
  3. Maceyka, M. et al. (2005) J. Biol. Chem. 280:37118.
Entrez Gene IDs
56848 (Human); 56632 (Mouse); 308589 (Rat)
Alternate Names
EC 2.7.1.91; SK 2; SK2; sphingosine kinase 2; sphingosine kinase type 2 isoform; SPHK2; SPK 2; SPK2

Citation for Recombinant Human Sphingosine Kinase 2/SPHK2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism
    Authors: N Turner, XY Lim, HD Toop, B Osborne, AE Brandon, EN Taylor, CE Fiveash, H Govindaraj, JD Teo, HP McEwen, TA Couttas, SM Butler, A Das, GM Kowalski, CR Bruce, KL Hoehn, T Fath, C Schmitz-Pe, GJ Cooney, MK Montgomery, JC Morris, AS Don
    Nat Commun, 2018-08-21;9(1):3165.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay

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