Recombinant Human Stabilin-2 His-tag Protein, CF Summary
Ser20-Thr2458, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Stabilin-2 His-tag Protein (Catalog # 11086-S2) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED50 of 1.50-7.50 ng/mL.
2 μg/lane of Recombinant Human Stabilin‑2 His-tag Protein (Catalog # 11086-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 260-300 kDa.
Stabilin-2, also known as HARE (Hyaluronic Acid Receptor for Endocytosis) and FEEL2 (Fasciclin, EGF-like, laminin type EGF-like, and Link domain containing scavenger receptor 2), is a type I transmembrane multidomain protein that is the closest homolog of stabilin1. It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrincoated pits (4). Stabilin2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation endproduct (AGE), collagen Nterminal propeptides and acetylated LDL (4-7). Stabilin-2 has been found to regulate the cancer progression, and high expression of Stabilin-2 was reported to predict poor tumor prognosis in NSCLC (8).
- Politz, O. et al. (2002) Biochem. J. 362:155.
- Harris, E.N. and F. Cabral (2019) Biomolecules 9:273.
- Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
- Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
- Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
- Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
- Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
- Yong, J. et al. (2021) Bioengineered 12:3426.
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