Recombinant Human TAFA3/FAM19A3 Protein, CF

Catalog # Availability Size / Price Qty
4677-TA-050
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Recombinant Human TAFA3/FAM19A3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human TAFA3/FAM19A3, immobilized at 4 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Source
E. coli-derived human TAFA3/FAM19A3 protein
Ala31-Arg133
Accession #
N-terminal Sequence
Analysis
Ala31
Predicted Molecular Mass
11 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4677-TA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAFA3/FAM19A3

TAFA3, also called FAM19A3, is a member of the FAM19/TAFA family of small secreted proteins (1). Human TAFA3 has a molecular weight of approximately 15 kDa. The mature protein is 103 amino acids (aa) in length and shares 93% aa sequence identity with the mouse and rat orthologs (1). Alternative exon splicing causes a reading frame shift at aa 89, creating a 169 aa isoform with an altered C-terminal region (1). TAFA3 is specifically expressed in the brain with the highest levels being detected in parietal cortex and basal ganglia (1). TAFA proteins display chemokine-like signatures and appear to be distantly related to CCL3/ MIP-1 alpha as they share a common CC motif (1). TAFA3 has been suggested to be involved in transmitting the photoperiodically-controlled Melatonin signal from the pars tuberalis (2). TAFA3 mRNA levels in the pars tuberalis vary between night and day and correspond to plasma Melatonin levels (2). Additionally, these day/night expression differences are not observed in mice that lack Melatonin type 1 receptors (2). Interestingly, in other brain regions, such as the hippocampus and the ventral posterior nucleus of the thalamus, TAFA3 expression does not appear to be mediated by Melatonin, suggesting expression of this protein is controlled by region-specific mechanisms (2).

References
  1. Tom Tang, Y. et al. (2004) Genomics 83:727.
  2. Fischer, C. et al. (2012) Gen. Comp. Endocrinol. 177:98.
Long Name
Family with Sequence Similarity 19, Member A3
Entrez Gene IDs
284467 (Human); 329731 (Mouse)
Alternate Names
Chemokine-like protein TAFA-3; FAM19A3; family with sequence similarity 19 (chemokine (C-C motif)-like), member A3; MGC138473; TAFA3; TAFA-3

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