Recombinant Human TDP-43/TARDBP Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 5 mM DTT, 20% (v/v) Glycerol, 0.4 M Urea|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates--along with a truncated 20-28 kDa form--in the Ubiquitin-containing inclusion bodies. TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis. Human TDP-43 is 96% identical to the mouse ortholog.
- Buratti E. et al. (2001) EMBO J. 20: 1774
- Chen-Plotkin A.S. et al. (2010) Nat. Rev. Neurol. 6: 211
- Johnson B.S. et al. (2009) J. Biol. Chem. 284: 20329
- Strong M.J. et al. (2007) Mol. Cell Neurosci. 35: 320
Why is this protein supplied with 0.4 M urea in the formulation buffer?
Without the presence of urea, this protein would be insoluble. This is partially due to the expression system and the nature of the protein. Any attempt to dilute the urea down will cause the protein to precipitate.
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