Recombinant Human TDP-43/TARDBP Protein, CF

Catalog # Availability Size / Price Qty
AP-190-100
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human TDP-43/TARDBP Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for in vitro use depends on the specific application.
Source
E. coli-derived human TDP-43/TARDBP protein
Accession #
Predicted Molecular Mass
45 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-190

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 5 mM DTT, 20% (v/v) Glycerol, 0.4 M Urea
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TDP-43/TARDBP

TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing.  TDP-43 is aberrantly expressed in most forms of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration.  In these cases it is absent from the nucleus and accumulates--along with a truncated 20-28 kDa form--in the Ubiquitin-containing inclusion bodies.  TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis.  Human TDP-43 is 96% identical to the mouse ortholog.

References
  1. Buratti E. et al. (2001) EMBO J20: 1774
  2. Chen-Plotkin A.S. et al. (2010) Nat. Rev. Neurol6: 211
  3. Johnson B.S. et al. (2009) J. Biol. Chem284: 20329
  4. Strong M.J. et al. (2007) Mol. Cell Neurosci35: 320
 
Long Name
TAR DNA-binding Protein 43
Entrez Gene IDs
23435 (Human)
Alternate Names
ALS10; ALS10TAR DNA-binding protein-43; TAR DNA binding protein; TARDBP; TDP43; TDP-43; TDP-43TAR DNA-binding protein 43

FAQs

  1. Why is this protein supplied with 0.4 M urea in the formulation buffer?

    • Without the presence of urea, this protein would be insoluble. This is partially due to the expression system and the nature of the protein. Any attempt to dilute the urea down will cause the protein to precipitate.

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