Recombinant Human TEM8/ANTXR1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
3886-AR-050
Recombinant Human TEM8/ANTXR1 His-tag Protein, CF Binding Activity
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Recombinant Human TEM8/ANTXR1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Anthrax Protective Antigen is coated at 1.5 μg/mL (100 μL/well), the concentration of Recombinant Human TEM8/ANTXR1 that produces 50% optimal binding response is 0.6-3.6 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human TEM8/ANTXR1 protein
Glu33-Ser321, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln28 inferred from enzymatic pyroglutamate treatment revealing Gly29
Structure / Form
Monomer
Predicted Molecular Mass
34 kDa
SDS-PAGE
38-46 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3886-AR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When anthrax protective antigen (PA) is coated at 1.5 μg/mL, 100 μL/well, Recombinant Human TEM8/ANTXR1 (Catalog # 3886-AR) binds with an ED50 of 0.6-3.6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human TEM8/ANTXR1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 38 - 46 kDa and 36 - 43 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TEM8/ANTXR1

Anthrax toxin receptor 1 (ANTXR1), also known as Tumor endothelial marker 8 (TEM8), is a glycoprotein of the Anthrax Toxin Receptor family that is expressed by endothelial cells. Anthrax toxin receptor 1 contains a 289 amino acid (aa) extracellular domain, a 21 aa transmembrane domain, and a 222 aa cytoplasmic domain. Type I transmembrane isoforms of 564 aa (80‑85 kDa) and 368 aa (60 kDa) and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) are differentially expressed. All diverge at the C-terminal end but share the N-terminal extracellular domain (1). The extracellular domain shares structural similarity with von Willebrand factor type (vWFA) domains, which are characterized by their interactions with ECM components (2, 3). The extracellular domain is involved in reorganization of cell actin cytoskeleton (2, 3). Anthrax Receptor 1 binds Anthrax Protective Antigen with lesser affinity that Anthrax Receptor 2 and induces toxin internalization (4). Anthrax toxin receptor 1 has been implicated in tumor angiogenesis, as its expression has been shown to up-regulate in tumor blood vessels and is characterized as a tumor endothelial marker (5). ANTXR-1 was reported to be an amplifier of Wnt signaling in tumor microenvironment (6). Additionally, Anthrax toxin receptor 1 serves as the receptor for Seneca Valley virus, an oncolytic picornavirus affecting neuroendocrine cancers (7). Human ANTXR1 shares 99% aa identity with mouse and rat and 92% identity with dog and chick ANTXR1 within the extracellular domain.

References
  1. Bradley, Kenneth A, et al. (2001) Nature 414:225.
  2. Hotchkiss, K. et al. (2004). Experimental Cell Research. 305:133.
  3. Whittaker, C. and Hynes, R. (2002). Mol Biol Cell. 13:3369.
  4. Sheng, Fu. et al. (2010) PLOS One. 5(6): e11203.
  5. Carson-Walter, EB. et al. (2001). Cancer Res. 18:6649.
  6. Verma, K. et al. (2011) PLOS One. 6(8):e22334.
  7. Miles, L. et al. (2017). J Clin Invest. 8:2957.
Long Name
Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1
Entrez Gene IDs
84168 (Human); 69538 (Mouse); 362393 (Rat)
Alternate Names
anthrax toxin receptor 1; ANTXR1; ATR2310008J16Rik; FLJ10601; FLJ11298; FLJ21776; TEM8; TEM82810405N18Rik; Tumor endothelial marker 8

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