Recombinant Human TFF1 Protein, CF

Catalog # Availability Size / Price Qty
8289-TF-050
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Recombinant Human TFF1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF1 can effectively induce ERK1/2 phosphorylation.
Source
Chinese Hamster Ovary cell line, CHO-derived human TFF1 protein
Glu25-Phe84 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
7.5 kDa
SDS-PAGE
12-15 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8289-TF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFF1

Trefoil Factor 1 (TFF1), also known as pS2, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF1 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). Mature human TFF1 shares 67% amino acid sequence identity with mouse and rat TFF1. It is expressed by goblet cells of the gastric and intestinal mucosa and by conjunctival goblet cells (2-5). TFF1 is a copper-binding protein that can form disulfide-linked homodimers, associate into disulfide-linked complexes with Gastrokine 2, and form non-covalent complexes with the mucin MUC5AC (4, 6-8). Copper enhances TFF1 homodimerization as well as its ability to promote epithelial cell motility, wound healing, and the colonization of H. pylori in stomach and colon epithelia (9, 10). TFF1 is down-regulated during the progression from gastritis to gastric dysplasia to gastric cancer, although it is up-regulated in breast and prostate cancers (11-13). TFF1 inhibits the formation of calcium oxalate crystals, and its excretion in the urine is reduced in patients with kidney stones (14).

References
  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. Madsen, J. et al. (2007) J. Histochem. Cytochem. 55:505.
  3. Rio, M.C. et al. (1988) Science 241:705.
  4. Newton, J.L. et al. (2000) Gut 46:312.
  5. Langer, G. et al. (1999) Invest. Opthalmol. Visual Sci. 40:2220.
  6. Chadwick, M.P. et al. (1997) Biochem. Biophys. Res. Commun. 327:117.
  7. Westley, B.R. et al. (2005) Biochemistry 44:7967.
  8. Ruchaud-Sparagano, M.H. et al. (2004) Cell. Mol. Life Sci. 61:1946.
  9. Tosco, A. et al. (2010) Cell. Mol. Life Sci. 67:1943.
  10. Montefusco, S. et al. (2013) PLoS ONE 8:e79455.
  11. Soutto, M. et al. (2011) J. Clin. Invest. 121:1753.
  12. Kida, N. et al. (1989) Cancer Res. 49:3494.
  13. Vestergaard, E.M. et al. (2006) Clin. Cancer Res. 12:807.
  14. Chutipongtanate, S. et al. (2005) J. Clin. Invest. 115:3613.
Long Name
Trefoil Factor 1
Entrez Gene IDs
7031 (Human); 100041004 (Mouse); 117270 (Rat)
Alternate Names
BCEI; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; PNR-2; Polypeptide P1.A; Protein pS2; PS2; TFF1; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A

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