Recombinant Human TFF3 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human TFF3 Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF3 can effectively induce ERK1/2 phosphorylation.
Chinese Hamster Ovary cell line, CHO-derived human TFF3 protein
Glu22-Phe80 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
7.4 kDa
7-11 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TFF3

Trefoil Factor 3 (TFF3), also known as Intestinal Trefoil Factor (ITF) and P1.B, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF3 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2 (2, 3). TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach (4-8). Following secretion, TFF3 can be retained in the overlying mucous layer (7). TFF3 is also expressed by chondrocytes during bone development (9). Mature human TFF3 shares 76% amino acid sequence identity with mouse and rat TFF3. TFF3 is up-regulated in response to a range of gastrointestinal epithelial disruptions (8, 10). It promotes epithelial wound healing by inducing the migration of biliary, bronchial, and intestinal epithelial cells (5, 10-12). TFF3 up-regulation is associated with and enhances tumor cell invasion and metastasis (6, 13). It supports hypoxia-induced VEGF up-regulation in tumor cells and also promotes angiogenesis in non-tumor environments (14, 15). Over-expression of TFF3 in type 2 diabetic mouse liver has been shown to improve glucose tolerance and insulin sensitivity (16).

  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. Albert, T.K. et al. (2010) J. Proteome Res. 9:3108.
  3. Yu, H. et al. (2011) PLoS ONE 6:e20334.
  4. LeSimple, P. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:296.
  5. Nozaki, I. et al. (2004) Am. J. Pathol. 165:1907.
  6. Ahmed, A.R.H. et al. (2012) Am. J. Pathol. 180:904.
  7. Podolsky, D.K. et al. (1993) J. Biol. Chem. 268:6694.
  8. Peitz, U. et al. (2004) Peptides 25:771.
  9. Bijelic, N. et al. (2013) Acta Histochem. 115:204.
  10. Paulsen, F.P. et al. (2008) J. Biol. Chem. 283:13418.
  11. Oertel, M. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:418.
  12. Dignass, A. et al. (1994) J. Clin. Invest. 94:376.
  13. Rivat, C. et al. (2005) Cancer Res. 65:195.
  14. Rodrigues, S. et al. (2003) FASEB J. 17:7.
  15. Guleng, B. et al. (2012) Mol. Biol. Rep. 39:4127.
  16. Xue, Y. et al. (2013) PLoS ONE 8:e75240.
Long Name
Trefoil Factor 3
Entrez Gene IDs
7033 (Human); 21786 (Mouse); 25563 (Rat)
Alternate Names
HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal)

Citation for Recombinant Human TFF3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair
    Authors: K Krüger, S Schmid, F Paulsen, A Ignatius, P Klinger, T Hotfiel, B Swoboda, K Gelse
    Int J Mol Sci, 2019;20(17):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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