Recombinant Human Thrombospondin-1 Protein, CF

Catalog # Availability Size / Price Qty
3074-TH-050
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (2)

Recombinant Human Thrombospondin-1 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 2 x 104 cells/well are added to DTT treated THBS-1 coated plates (10 µg/mL, 100 µL/well), > 50% will adhere after 1 hour at 37 °C.
Source
Mouse myeloma cell line, NS0-derived human Thrombospondin-1 protein
Asn19-Pro1170, with Thr523Ala substitution and a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Asn19
Predicted Molecular Mass
129 kDa
SDS-PAGE
135 - 165 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3074-TH

Formulation Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150-180 kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. Within the Thrombospondin family, TSP-2 is also homotrimeric, while TSP-3, -4, and -5/COMP are homopentameric. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1 domains, 2 EGF-like domains, and 8 TSP type-3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature human TSP-1 shares 95% amino acid sequence identity with mouse and rat TSP-1. TSP-1 counteracts the angiogenic, hypotensive, and anti-thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4-6). TSP-1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP-1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP-1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

References
  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  3. Roberts, D.D. et al. (2012) Matrix Biol. 31:162.
  4. Gupta, K. et al. (1999) Angiogenesis 3:147.
  5. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923.
  6. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79.
  7. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  8. Isenberg, J.S. et al. (2008) Blood 111:613.
  9. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775.
  10. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
  11. Grimbert, P. et al. (2006) J. Immunol. 177:3534.
  12. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991.
  13. Xu, J. et al. (2010) Nat. Neurosci. 13:22.
  14. Eroglu, C. et al. (2009) Cell 139:380.
  15. Blake, S.M. et al. (2008) EMBO J. 27:3069.
  16. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.
Entrez Gene IDs
7057 (Human); 21825 (Mouse)
Alternate Names
THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1

Citations for Recombinant Human Thrombospondin-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis
    Authors: S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith
    Microorganisms, 2019;7(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses
    Authors: A Ojha, A Bhasym, S Mukherjee, GK Annarapu, T Bhakuni, I Akbar, T Seth, NK Vikram, S Vrati, A Basu, S Bhattachar, P Guchhait
    EBioMedicine, 2019;39(0):332-347.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment
    Authors: J Guillon, C Petit, M Moreau, B Toutain, C Henry, H Roché, N Bonichon-L, JP Salmon, J Lemonnier, M Campone, V Verrièle, E Lelièvre, C Guette, O Coqueret
    Cell Death Dis, 2019;10(3):199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer's disease model.
    Authors: Dong Hyun Kim, Hoon Lim, Dahm Lee, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Su Oh, Hyun Ho Hwang, Hong Bae Jeon
    Scientific Reports, 2018;0(0):2045-2322.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells
    Authors: G Inana, C Murat, W An, X Yao, IR Harris, J Cao
    J Transl Med, 2018;16(1):63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. SCO-spondin oligopeptide inhibits angiogenesis in glioblastoma
    Authors: R Bibes, S Gobron, F Vincent, C Mélin, N Vedrenne, A Perraud, F Labrousse, MO Jauberteau, F Lalloué
    Oncotarget, 2017;8(49):85969-85983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Decorin-inducible Peg3 Evokes Beclin 1-mediated Autophagy and Thrombospondin 1-mediated Angiostasis
    Authors: A Torres, MA Gubbiotti, RV Iozzo
    J. Biol. Chem, 2017;292(12):5055-5069.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer
    Authors: K Endt, J Goepfert, A Omlin, A Athanasiou, P Tennstedt, A Guenther, M Rainisio, DS Engeler, T Steuber, S Gillessen, T Joos, R Schiess
    PLoS ONE, 2017;12(8):e0181557.
    Species: Human
    Sample Types:
    Applications: ELISA (Standard)
  9. Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells
    Authors: C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating E2 Synthesis and Inhibiting Thrombospondin-1 (TSP-1)
    Authors: J Zhai, J Liu, X Cheng, S Li, Y Hong, K Sun, ZJ Chen, Y Du, W Li
    Sci Rep, 2017;7(1):3245.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Thrombospondin 1 Deficiency Ameliorates the Development of Adriamycin-Induced Proteinuric Kidney Disease
    PLoS ONE, 2016;11(5):e0156144.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
    Cell Death Dis, 2016;7(12):e2565.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The structural basis for CD36 binding by the malaria parasite
    Nat Commun, 2016;7(0):12837.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  14. The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components
    Invest Ophthalmol Vis Sci, 2016;57(11):4599-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein.
    Authors: Zheng Y, Zou F, Wang J, Yin G, Le V, Fei Z, Liu J
    J Biol Chem, 2015;290(14):8975-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
    Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
    Invest Ophthalmol Vis Sci, 2015;56(2):1051-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Thrombospondin 1 inhibits inflammatory lymphangiogenesis by CD36 ligation on monocytes.
    Authors: Cursiefen C, Maruyama K, Bock F, Saban D, Sadrai Z, Lawler J, Dana R, Masli S
    J. Exp. Med., 2011;208(5):1083-92.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010;85(7):487-93.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  19. Variable inhibition of thrombospondin 1 against liver and lung metastases through differential activation of metalloproteinase ADAMTS1.
    Authors: Lee YJ, Koch M, Karl D
    Cancer Res., 2010;70(3):948-56.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  20. Estrogen regulation of thrombospondin-1 in human breast cancer cells.
    Int. J. Cancer, 2009;125(0):1045.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human Thrombospondin-1 Protein, CF

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Recombinant Human Thrombospondin-1 Protein, CF
By Anonymous on 10/04/2019
Application: Immunoassay Standard

We used this protein for a sandwich ELISA in combination with mAb (MAB3074)) and pAb (AF3074). This combination works very well for detecting the TSP1 in human serum and plasma samples.


Recombinant Human Thrombospondin-1 Protein, CF
By Anonymous on 05/22/2018
Application: CellProlif