Best Seller

Recombinant Human Thrombospondin-1 Protein, CF

R&D Systems | Catalog # 3074-TH

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Human Thrombospondin-1 Protein (3074-TH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P07996

Applications

Bioactivity
View all Thrombospondin-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Thrombospondin-1 protein
Asn19-Pro1170, with Thr523Ala substitution and a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn19

Predicted Molecular Mass

129 kDa

SDS-PAGE

135-165 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human VEGF (Catalog # 8147-VE/CF) is coated at 1.00 μg/mL (100 μL/well), it binds to Recombinant Human Thrombospondin‑1. The ED50 for this binding is 100-600 ng/mL.

Reviewed Applications

Read 2 reviews rated 5 using 3074-TH in the following applications:

Formulation, Preparation, and Storage

3074-TH
Formulation Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150-180 kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. Within the Thrombospondin family, TSP-2 is also homotrimeric, while TSP-3, -4, and -5/COMP are homopentameric. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1 domains, 2 EGF-like domains, and 8 TSP type-3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature human TSP-1 shares 95% amino acid sequence identity with mouse and rat TSP-1. TSP-1 counteracts the angiogenic, hypotensive, and anti-thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4-6). TSP-1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP-1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP-1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

References

  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  3. Roberts, D.D. et al. (2012) Matrix Biol. 31:162.
  4. Gupta, K. et al. (1999) Angiogenesis 3:147.
  5. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923.
  6. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79.
  7. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  8. Isenberg, J.S. et al. (2008) Blood 111:613.
  9. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775.
  10. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
  11. Grimbert, P. et al. (2006) J. Immunol. 177:3534.
  12. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991.
  13. Xu, J. et al. (2010) Nat. Neurosci. 13:22.
  14. Eroglu, C. et al. (2009) Cell 139:380.
  15. Blake, S.M. et al. (2008) EMBO J. 27:3069.
  16. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human); 21825 (Mouse)

Gene Symbol

THBS1

UniProt

P07996

Additional Thrombospondin-1 Products

Product Documents for Recombinant Human Thrombospondin-1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Thrombospondin-1 Protein, CF

For research use only

Citations for Recombinant Human Thrombospondin-1 Protein, CF

Customer Reviews for Recombinant Human Thrombospondin-1 Protein, CF (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human Thrombospondin-1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Human Thrombospondin-1 Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 10/04/2019
    We used this protein for a sandwich ELISA in combination with mAb (MAB3074)) and pAb (AF3074). This combination works very well for detecting the TSP1 in human serum and plasma samples.
    Recombinant Human Thrombospondin-1 Protein, CF 3074-TH
  • Recombinant Human Thrombospondin-1 Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/22/2018

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes