Recombinant Human Thrombospondin-2 Protein, CF

Catalog # Availability Size / Price Qty
1635-T2-050
Recombinant Human Thrombospondin-2 Protein Bioactivity
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Product Details
Citations (7)
FAQs
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Recombinant Human Thrombospondin-2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.

The ED50 for this effect is 0.07-0.7 μg/mL.

Source
Mouse myeloma cell line, NS0-derived human Thrombospondin-2 protein
Gly19-Ile1172, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Gly19
Predicted Molecular Mass
129 kDa
SDS-PAGE
170-176 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1635-T2

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Thrombospondin-2 Protein Bioactivity View Larger

Recombinant Human Thrombospondin-2 His-tag (Catalog # 1635-T2) supports the adhesion of SVEC4-10 vascular endothelial cells. The ED50 for this effect is 0.07-0.7 μg/mL.

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Background: Thrombospondin-2

Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein that modulates cellular interactions with extracellular matrix. Thrombospondin-1 and -2 constitute subgroup A thrombospondin family members and form disulfide-linked homotrimers, whereas Thrombospondin-3, -4, and -5/COMP constitute subgroup B and form homopentamers (1‑4). The human TSP-2 cDNA encodes a 1172 amino acid (aa) precursor that includes an 18 aa signal sequence followed by an N-terminal heparin‑binding domain, an oligomerization motif, one vWF-C domain, three TSP type-1 repeats, three EGF-like repeats, seven TSP type-3 repeats, and a lectin-like TSP C‑terminal domain (5). Human TSP-2 shares 88‑90% aa sequence identity with bovine, mouse, and rat TSP-2. Within the TSP type-3 repeats and TSP C-terminal domain, human TSP-2 shares 80% aa sequence identity with human TSP-1 and approximately 60% aa sequence identity with human TSP-3, -4, and -5/COMP. TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult (6, 7). Trimerization of TSP-2 is required for the calcium-dependent cell attachment and spreading functions, while the heparin‑binding domain is responsible for the destabilization of focal adhesion sites (8‑10). The heparin‑binding domain also mediates binding to Integrins alpha 3 beta 1 and alpha 6 beta 1 on microvascular endothelial cells (EC) and Integrin alpha 4 beta 1 on large blood vessel EC (11, 12). A fragment of TSP-2 (heparin‑binding domain, oligomerization motif, and vWF-C domain) promotes EC survival, proliferation, and chemotaxis (11). Inclusion of the three TSP type-1 domains results in a molecule that inhibits VEGF-induced EC migration and vascular tube formation (13, 14). In vivo, full length TSP-2 blocks tumor angiogenesis and induces vascular EC apoptosis (13, 15). HPRG functions as an apparent decoy receptor by preventing interaction of TSP-2 with CD36 on macrophages and microvasculature EC (14). TSP-2 also binds MMP-2 and facilitates MMP-2 clearance by the scavenger receptor LRP (16).

References
  1. Elzie, C.A. and J.E. Murphy-Ullrich (2004) Int. J. Biochem. Cell Biol. 36:1090.
  2. Armstrong, L.C. and P. Bornstein (2003) Matrix Biol. 22:63.
  3. Murphy-Ullrich, J.E. (2001) J. Clin. Invest. 107:785.
  4. Bornstein, P. and E.H. Sage (2002) Curr. Opin. Cell Biol. 14:608.
  5. LaBell, T.L. and P.H. Byers (1993) Genomics 17:225.
  6. Kyriakides, T.R. et al. (1998) J. Histochem. Cytochem. 46:1007.
  7. Kyriakides, T.R. et al. (1998) J. Cell Biol. 140:419.
  8. Anilkumar, N. et al. (2002) J. Cell Sci. 115:2357.
  9. Misenheimer, T.M. et al. (2003) Biochemistry 42:5125.
  10. Murphy-Ullrich, J.E. et al. (1993) J. Biol. Chem. 268:26784.
  11. Calzada, M.J. et al. (2004) Circ. Res. 94:462.
  12. Calzada, M.J. et al. (2003) J. Biol. Chem. 278:40679.
  13. Noh, Y.-H. et al. (2003) J. Invest. Dermatol. 121:1536.
  14. Simantov, R. et al. (2005) Matrix Biol. 24:27.
  15. Streit, M. et al. (1999) Proc. Natl. Acad. Sci. 96:14888.
  16. Yang, Z. et al. (2001) J. Biol. Chem. 276:8403.
Entrez Gene IDs
7058 (Human)
Alternate Names
THBS2; thrombospondin 2; Thrombospondin2; Thrombospondin-2; TSP-2; TSP2thrombospondin-2

Citations for Recombinant Human Thrombospondin-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Thrombospondin-2 stimulates MMP-9 production and promotes osteosarcoma metastasis via the PLC, PKC, c-Src and NF-&kappaB activation
    Authors: JF Liu, PC Chen, TM Chang, CH Hou
    J Cell Mol Med, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells
    Authors: G Inana, C Murat, W An, X Yao, IR Harris, J Cao
    J Transl Med, 2018;16(1):63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels
    Authors: F Roudnicky, SY Yoon, S Poghosyan, S Schwager, C Poyet, G Vella, SB Bachmann, S Karaman, JW Shin, VI Otto, M Detmar
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  4. Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure
    Authors: S Wang, T Hu, Z Wang, N Li, L Zhou, L Liao, M Wang, L Liao, H Wang, L Zeng, C Fan, H Zhou, K Xiong, J Huang, D Chen
    PLoS ONE, 2017;12(9):e0185388.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
    Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
    J Hematol Oncol, 2017;10(1):33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome.
    Authors: Hellbach N, Weise S, Vezzali R, Wahane S, Heidrich S, Roidl D, Pruszak J, Esser J, Vogel T
    Hum Mol Genet, 2014;23(23):6177-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013;497(7449):369-73.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay

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Recombinant Human Thrombospondin-2 Protein, CF
By Anonymous on 09/23/2019
Application: Immunoassay Standard

We used this protein for developing an ELISA in combination with mAb (cat. MAB16351)) and pAb (cat. AF1635). This combination works well detecting TSP2 in human serum.