Recombinant Human Thrombospondin-2 Protein, CF Summary
The ED50 for this effect is 0.07-0.7 μg/mL.
Gly19-Ile1172, with a C-terminal 10-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MES and NaCl.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human Thrombospondin-2 His-tag (Catalog # 1635-T2) supports the adhesion of SVEC4-10 vascular endothelial cells. The ED50 for this effect is 0.07-0.7 μg/mL.
Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein that modulates cellular interactions with extracellular matrix. Thrombospondin-1 and -2 constitute subgroup A thrombospondin family members and form disulfide-linked homotrimers, whereas Thrombospondin-3, -4, and -5/COMP constitute subgroup B and form homopentamers (1‑4). The human TSP-2 cDNA encodes a 1172 amino acid (aa) precursor that includes an 18 aa signal sequence followed by an N-terminal heparin‑binding domain, an oligomerization motif, one vWF-C domain, three TSP type-1 repeats, three EGF-like repeats, seven TSP type-3 repeats, and a lectin-like TSP C‑terminal domain (5). Human TSP-2 shares 88‑90% aa sequence identity with bovine, mouse, and rat TSP-2. Within the TSP type-3 repeats and TSP C-terminal domain, human TSP-2 shares 80% aa sequence identity with human TSP-1 and approximately 60% aa sequence identity with human TSP-3, -4, and -5/COMP. TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult (6, 7). Trimerization of TSP-2 is required for the calcium-dependent cell attachment and spreading functions, while the heparin‑binding domain is responsible for the destabilization of focal adhesion sites (8‑10). The heparin‑binding domain also mediates binding to Integrins alpha 3 beta 1 and alpha 6 beta 1 on microvascular endothelial cells (EC) and Integrin alpha 4 beta 1 on large blood vessel EC (11, 12). A fragment of TSP-2 (heparin‑binding domain, oligomerization motif, and vWF-C domain) promotes EC survival, proliferation, and chemotaxis (11). Inclusion of the three TSP type-1 domains results in a molecule that inhibits VEGF-induced EC migration and vascular tube formation (13, 14). In vivo, full length TSP-2 blocks tumor angiogenesis and induces vascular EC apoptosis (13, 15). HPRG functions as an apparent decoy receptor by preventing interaction of TSP-2 with CD36 on macrophages and microvasculature EC (14). TSP-2 also binds MMP-2 and facilitates MMP-2 clearance by the scavenger receptor LRP (16).
- Elzie, C.A. and J.E. Murphy-Ullrich (2004) Int. J. Biochem. Cell Biol. 36:1090.
- Armstrong, L.C. and P. Bornstein (2003) Matrix Biol. 22:63.
- Murphy-Ullrich, J.E. (2001) J. Clin. Invest. 107:785.
- Bornstein, P. and E.H. Sage (2002) Curr. Opin. Cell Biol. 14:608.
- LaBell, T.L. and P.H. Byers (1993) Genomics 17:225.
- Kyriakides, T.R. et al. (1998) J. Histochem. Cytochem. 46:1007.
- Kyriakides, T.R. et al. (1998) J. Cell Biol. 140:419.
- Anilkumar, N. et al. (2002) J. Cell Sci. 115:2357.
- Misenheimer, T.M. et al. (2003) Biochemistry 42:5125.
- Murphy-Ullrich, J.E. et al. (1993) J. Biol. Chem. 268:26784.
- Calzada, M.J. et al. (2004) Circ. Res. 94:462.
- Calzada, M.J. et al. (2003) J. Biol. Chem. 278:40679.
- Noh, Y.-H. et al. (2003) J. Invest. Dermatol. 121:1536.
- Simantov, R. et al. (2005) Matrix Biol. 24:27.
- Streit, M. et al. (1999) Proc. Natl. Acad. Sci. 96:14888.
- Yang, Z. et al. (2001) J. Biol. Chem. 276:8403.
Citations for Recombinant Human Thrombospondin-2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 9
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Multi-scale integrative analyses identify THBS2+ cancer-associated fibroblasts as a key orchestrator promoting aggressiveness in early-stage lung adenocarcinoma
Authors: H Yang, B Sun, L Fan, W Ma, K Xu, SRR Hall, Z Wang, RA Schmid, RW Peng, TM Marti, W Gao, J Xu, W Yang, F Yao
Sample Types: In Vivo
Applications: In Vivo
Thrombospondin-2 stimulates MMP-9 production and promotes osteosarcoma metastasis via the PLC, PKC, c-Src and NF-&kappaB activation
Authors: JF Liu, PC Chen, TM Chang, CH Hou
J Cell Mol Med, 2020;0(0):.
Sample Types: Whole Cells
Applications: Cell Culture
Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine
Authors: NMP Cartee, SJ Lee, SG Keep, MM Wang
PLoS ONE, 2020;15(11):e0242376.
Sample Types: Cell Lysates
Applications: Western Blot Control
RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells
Authors: G Inana, C Murat, W An, X Yao, IR Harris, J Cao
J Transl Med, 2018;16(1):63.
Sample Types: Whole Cells
Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels
Authors: F Roudnicky, SY Yoon, S Poghosyan, S Schwager, C Poyet, G Vella, SB Bachmann, S Karaman, JW Shin, VI Otto, M Detmar
Sample Types: Tissue Homogenates
Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure
Authors: S Wang, T Hu, Z Wang, N Li, L Zhou, L Liao, M Wang, L Liao, H Wang, L Zeng, C Fan, H Zhou, K Xiong, J Huang, D Chen
PLoS ONE, 2017;12(9):e0185388.
Sample Types: Whole Cells
Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
J Hematol Oncol, 2017;10(1):33.
Sample Types: Whole Tissue
Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome.
Authors: Hellbach N, Weise S, Vezzali R, Wahane S, Heidrich S, Roidl D, Pruszak J, Esser J, Vogel T
Hum Mol Genet, 2014;23(23):6177-90.
Sample Types: Whole Cells
Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
Sample Types: Protein
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We used this protein for developing an ELISA in combination with mAb (cat. MAB16351)) and pAb (cat. AF1635). This combination works well detecting TSP2 in human serum.