Recombinant Human THSD7A Protein, CF

Catalog # Availability Size / Price Qty
9524-TH-050
Recombinant Human THSD7A Protein Bioactivity
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Recombinant Human THSD7A Protein, CF Summary

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.6 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human THSD7A protein
Ala48-Trp1607, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala48
Predicted Molecular Mass
176 kDa
SDS-PAGE
160-265 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9524-TH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human THSD7A Protein Bioactivity View Larger

Immobilized Recombinant Human THSD7A (Catalog # 9524-TH) supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50for this effect is 0.1-0.6 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: THSD7A

Thrombospondin type I domain containing 7A, also known as THSD7A, is an approximately 250 kDa type I membrane protein (1). THSD-7A has a large extracellular domain containing ten thrombospondin type 1 repeats, six WSXW motifs, one RGD motif, and fourteen predicted N‑glycosylated sites (1). Mature THSD7A shares 91% and 92% amino acid sequence identity with mouse and rat, respectively. THSD7A is expressed in podocytes, glomerular endothelial cells and mesangial cells (2). It is a novel neural protein known to affect endothelial migration and vascular patterning during development (1, 3). Soluble THSD7A promotes endothelial filopodia formation and focal adhesion assembly and induces FAK-dependent signaling during angiogenesis (1). THSD7A can co-localize with aVb3 integrin in HUVECs (3). Additionally, most recent study has indicated that THSD7A is associated with obesity (4).

References
  1. Kuo, M.W. et al. (2011) PLoS One 6: e29000.
  2. Tomas, N.M. et al. (2014) N. Engl. J. Med. 371:2277.
  3. Wang, C.H. et al. (2010) J. Cell Physiol. 222: 685.
  4. Nizamuddin, S. et al. (2015) Int. J. Obes. (Lond.) 39:1662.
Long Name
Thrombospondin, Type I, Domain Containing 1
Entrez Gene IDs
221981 (Human); 330267 (Mouse); 500032 (Rat)
Alternate Names
Gm837; KIAA0960; thrombospondin, type I, domain containing 7A; THSD7A

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