Recombinant Human THSD7A Protein, CF Summary
Ala48-Trp1607, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Human THSD7A (Catalog # 9524-TH) supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50for this effect is 0.1-0.6 μg/mL.
Thrombospondin type I domain containing 7A, also known as THSD7A, is an approximately 250 kDa type I membrane protein (1). THSD-7A has a large extracellular domain containing ten thrombospondin type 1 repeats, six WSXW motifs, one RGD motif, and fourteen predicted N‑glycosylated sites (1). Mature THSD7A shares 91% and 92% amino acid sequence identity with mouse and rat, respectively. THSD7A is expressed in podocytes, glomerular endothelial cells and mesangial cells (2). It is a novel neural protein known to affect endothelial migration and vascular patterning during development (1, 3). Soluble THSD7A promotes endothelial filopodia formation and focal adhesion assembly and induces FAK-dependent signaling during angiogenesis (1). THSD7A can co-localize with aVb3 integrin in HUVECs (3). Additionally, most recent study has indicated that THSD7A is associated with obesity (4).
- Kuo, M.W. et al. (2011) PLoS One 6: e29000.
- Tomas, N.M. et al. (2014) N. Engl. J. Med. 371:2277.
- Wang, C.H. et al. (2010) J. Cell Physiol. 222: 685.
- Nizamuddin, S. et al. (2015) Int. J. Obes. (Lond.) 39:1662.
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