Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human TIM-1/KIM-1/HAVCR (175 aa) Fc Chimera Protein Bioactivity.
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Product Details

Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.500-10.0 μg/mL.
Human embryonic kidney cell, HEK293-derived human TIM-1/KIM-1/HAVCR protein
Accession # Q96D42.3
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
56 kDa
113-132 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.500-10.0 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human TIM-1/KIM-1/HAVCR (175 aa) Fc Chimera Protein (Catalog # 11273-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 113-132 kDa and 230-260 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.



T cell-immunoglobulin and mucin domain 1 (TIM-1), also known as KIM1 and HAVcr1, type I transmembrane glycoprotein in the TIM subfamily of immunoglobulin (Ig) superfamily molecules. In human, the TIM family is comprised of 3 family members and is involved in the regulation of Th1 and Th2‑cell‑mediated immune responses (1). Mature TIM-1 consists of an extracellular domain (ECD) with one V‑type Ig‑like domain and a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain. Within the ECD, human TIM-1 shares 39% amino acid sequence identity with mouse TIM-1. Multiple TIM-1 variants can be produced either through polymorphisms or alternative splicing primarily resulting in deletions in the mucin domain (2). A soluble form of TIM-1, detectable in the urine and in circulation, can be generated through ectodomain cleavage mediated by MMP3 (3, 4). TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (5-7). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (8-10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (5, 11). TIM-1 serves as a receptor for phosphatidylserine, and its interaction with LMIR5 enables TIM-1 to mediate the phagocytosis of apoptotic cells (12). TIM-1 also serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus, Marburgvirus and has been indicated as a possible receptor for SARS-CoV-2 (13-15).

  1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
  2. Freeman, G.J. et al. (2010) Immunol. Rev. 235:172.
  3. Lim, A.I. et al. (2012) Int. J. Biochem. Cell Biol. 44:1040.
  4. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  5. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
  6. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
  7. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  8. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
  9. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
  10. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
  11. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  12. Kobayashi, N. et al. (2007) Immunity 27:927.
  13. Tami, C. et al. (2007) J. Virol. 81:3437.
  14. Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
  15. Ichimura, T. et al. (2020) medRxiv


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