Recombinant Human TMEM119 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10313-TM-050
Recombinant Human TMEM119 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human TMEM119 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse TMEM119 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse TMEM119 protein
Human TMEM119
(Arg26-Met96)
Accession # Q4V9L6
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Arg26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
34 kDa
SDS-PAGE
37-57 kDa, under reducing conditions.

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10313-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human TMEM119 Fc Chimera (Catalog # 10313-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-57 kDa and 74-114 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMEM119

TMEM119, also known as osteoblast induction factor (OBIF), is a type 1a transmembrane protein and is frequently O-glycosylated at its N-terminal region (1, 2). TMEM119 is 283 amino acids (aa) in length and contains a 70 aa extracellular domain (ECD), a 20 aa helical transmembrane component, and a 165 aa cytoplasmic region. Human TMEM119 has 70% and 68% aa similarity to mouse and rat, respectively. TMEM119 is predominantly expressed in osteoblasts and is involved in the osteoblast differentiation and bone development by acting as a ligand (2, 3). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (AD) (4). TMEM119 has also been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (2, 3).

References
  1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
  4. Satoh, J. et al. (2016) Neuropathol. 36:39.
Long Name
Transmembrane Protein 119
Entrez Gene IDs
338773 (Human); 231633 (Mouse)
Alternate Names
OBIF; Osteoblast Induction Factor; TMEM119

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