Recombinant Human TMEM119 Fc Chimera Protein, CF Summary
|Human TMEM119 |
Accession # Q4V9L6.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human TMEM119 Fc Chimera (Catalog # 10313-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-57 kDa and 70-110 kDa, respectively.
TMEM119 (Transmembrane Protein 119, also known as Osteoblast
Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane
protein that is predominantly expressed in osteoblasts and is upregulated
during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell
line of microglia, and TMEM119 immunoreactivity is observed in a specific
subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's
disease (3). Mature human TMEM119 consists of a 71 amino acid (aa)
extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa
cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa
sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is
involved in the osteoblast differentiation and bone development by acting as a
ligand and has been reported to contribute to the proliferation, migration, and
invasion of osteosarcoma cells, as well as functioning as an oncogene in
osteosarcoma (3, 4).
- Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
- Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
- Satoh, J. et al. (2016) Neuropathol. 36:39.
- Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
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