Recombinant Human TMEM119 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10313-TM-050
Recombinant Human TMEM119 Fc Chimera Protein, CF SDS-PAGE
1 Image
Product Details
FAQs
Reviews

Recombinant Human TMEM119 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human TMEM119 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human TMEM119 protein
Human TMEM119
(Arg26-Met96)
Accession # Q4V9L6.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Arg26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
34 kDa
SDS-PAGE
37-57 kDa, under reducing conditions.

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10313-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE View Larger

2 μg/lane of Recombinant Human TMEM119 Fc Chimera (Catalog # 10313-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-57 kDa and 70-110 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TMEM119

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3).  Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).




References
  1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Satoh, J. et al. (2016) Neuropathol. 36:39.
  4. Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
Long Name
Transmembrane Protein 119
Entrez Gene IDs
338773 (Human); 231633 (Mouse)
Alternate Names
OBIF; Osteoblast Induction Factor; TMEM119

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human TMEM119 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human TMEM119 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human TMEM119 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review