Recombinant Human TMEM119 Fc Chimera Protein, CF Summary
|Human TMEM119 |
Accession # Q4V9L6
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human TMEM119 Fc Chimera (Catalog # 10313-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-57 kDa and 74-114 kDa, respectively.
TMEM119, also known as osteoblast induction factor (OBIF), is a type 1a transmembrane protein and is frequently O-glycosylated at its N-terminal region (1, 2). TMEM119 is 283 amino acids (aa) in length and contains a 70 aa extracellular domain (ECD), a 20 aa helical transmembrane component, and a 165 aa cytoplasmic region. Human TMEM119 has 70% and 68% aa similarity to mouse and rat, respectively. TMEM119 is predominantly expressed in osteoblasts and is involved in the osteoblast differentiation and bone development by acting as a ligand (2, 3). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (AD) (4). TMEM119 has also been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (2, 3).
- Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
- Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
- Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
- Satoh, J. et al. (2016) Neuropathol. 36:39.
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