Chinese Hamster Ovary cell line, CHO-derived human TMPRSS11A protein
Phe41-Ile421, with modifications in the non-catalytic chain and a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe41 & Ala149 (non-catalytic chain), Ile187 (catalytic chain)

Predicted Molecular Mass

17 kDa (non-catalytic), 29 kDa (catalytic)

SDS-PAGE

19-22 kDa (non-catalytic), 29-39 kDa (catalytic), & 51-56 kDa (intact), reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014).
The specific activity is >350 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human TMPRSS11A His-tag, CF

Recombinant Human TMPRSS11A His-tag Enzyme Activity.

Recombinant Human TMPRSS11a His-tag (Catalog # 11785-TP) is measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (ES014).

Formulation, Preparation, and Storage

11785-TP

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: TMPRSS11A

Recombinant Transmembrane protease serine 11a (TMPRSS11a), also known as Airway trypsin-like protease 1 (HATL1), Esophageal cancer-susceptibility gene 1 protein (ECRG1), and HESP is a member of the type II transmembrane serine protease (TTSP) peptidase S1 family from the human airway trypsin-like (HAT) subgroup (1). TMPRSS11a is expressed in select tissues including esophagus, lung, and aged tissue (2,3). Similar to other members of the TTSP family, TMPRSS11a is produced as a zymogen and is autoactivated into disulfide-linked non-catalytic chains composed of the TM and sea urchin sperm protein (SEA) domain and catalytic chains with a highly conserved serine protease (SP) domain (1,4). TMPRSS11a consists of three distinct regions, including an N-terminal cytoplasmic tail, a single-pass transmembrane domain, and an extracellular domain composed of the SEA domain and C-terminal serine protease domain (4). In human and mouse airways TMPRSS11A is expressed on the epithelial surface (5). Proteolytic cleavage of viral particle surface proteins by TMPRSS11a as a host protease is involved in facilitation of cell entry for influenza A viruses and coronaviruses (5-7). Carriers of the mutant alleles of TMPRSS11A are thought to have increased risk of severe COVID 19 in association with systemic inflammation, hypercoagulability, and organ damage (8). Inhibitors of TTSPs including TMPRSS11a present in the lung have been proposed as broad-spectrum antiviral agents (5). TMPRSS11a downregulation is reported to play a role in cancer as variants are linked to the risk of oral and esophageal squamous cell carcinomas (9-11) and TMPRSS11a was identified as a putative tumor suppressor in human esophageal cancers (12,13). TMPRSS11a is upregulated in aged skin and gingival tissue, inhibits cell migration through binding to integrin beta 1 via a putative RGD motif contained within the C-terminal trypsin-like serine proteinase domain to play a role in inducing cellular senescence and attenuate wound healing capacity (3,13) making it a target for skin wound healing during aging (3).

References

Wu, Q. et al. (2023) Biomedicines 11:1794.
Su, T. et al. (1998) Zhonghua Zhong Liu Za Zhi 20:254.
Fernandez, C. et al. (2021) FASEB J. 35:e21597.
Zhang, C. et al. (2020) J. Biol. Chem. 295:12686.
Fuentes-Prior, P. (2021) J. Biol. Chem. 296:100135.
Kam, Y.W. et al. (2009) PLoS ONE 4:e7870.
Zmora, P. et al. (2018) J. Biol. Chem. 293:13863.
Cekin, N. et al. (2025) Mol. Biol. Rep. 53:51.
Li, Y. et al. (2006) Carcinogenesis 27:798.
Bachmann, K. et al. (2009) J. Gastrointest. Surg. 13:181.
Rasool S, et al. (2013) Onkologie. 36:213.
Zhao, N. et al. (2004) J. Cell. Biochem. 92:65.
Zhao, N. et al. (2006) Exp. Biol. Med. 231:84.

Long Name

Transmembrane Serine Protease 11A

Alternate Names

ECRG1, HATL1, HESP

Entrez Gene IDs

339967 (Human)

Gene Symbol

TMPRSS11A

UniProt

KAI2534484.1

Additional TMPRSS11A Products

Product Documents for Recombinant Human TMPRSS11A His-tag, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human TMPRSS11A His-tag, CF

For research use only

Related Research Areas

Serine Proteases and Regulators

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Protocols

View specific protocols for Recombinant Human TMPRSS11A His-tag, CF (11785-TP):

Materials

Assay Buffer: 50 mM Tris, 0.05% BRIJ 35, pH 8.0
Recombinant Human TMPRSS11A His-tag (rhTMPRSS11A) (Catalog # 11785-TP)
Substrate: BOC-Gln-Ala-Arg-AMC (Catalog # ES014), 10 mM stock in DMSO
Black 96-well Plate
Plate Reader with Fluorescence Read Capability

Dilute rhTMPRSS11A to 2.0 µg/mL in Assay Buffer.
Dilute Substrate to 600 µM in Assay Buffer.
Load into a plate 50 µL of 2.0 µg/mL rhTMPRSS11A and start the reaction by adding 50 µL of 600 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 600 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
Calculate specific activity:

Specific Activity (pmol/min/µg) =	Adjusted V_max* (RFU/min) x Conversion Factor** (pmol/RFU)
	amount of enzyme (µg)

*Adjusted for Substrate Blank

**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC)

Per Well:

rhTMPRSS11A: 0.1 µg
Substrate: 300 µM

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Recombinant Human TMPRSS11A His-tag, CF

R&D Systems | Catalog # 11785-TP

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