Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Formulations:
Catalog # Availability Size / Price Qty
375-TEC-010
Product Details
Citations (10)
FAQs
Reviews (1)

Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.5‑6 ng/mL.
Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.
Source
E. coli-derived human TRAIL/TNFSF10 protein
Val114-Gly281, with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Val114
Structure / Form
Homotrimer
Predicted Molecular Mass
19 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

375-TEC

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, (NH4)2SO4 and ZnSO4 with BSA as a carrier protein.
Reconstitution Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 1 month, -20 to -70 °C under sterile conditions after reconstitution.

375-TEC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, (NH4)2SO4 and ZnSO4.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 1 month, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: TRAIL/TNFSF10

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is the most homologous to Fas ligand, sharing 28% aa sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% aa sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that transduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4). It was shown that homotrimeric TRAIL binds a zinc ion which is critical for the correct structure of the protein (5 - 6).

References
  1. Golstein, P. (1997) Current Biology 7:R750.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.
  5. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
  6. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
Long Name
TNF-related Apoptosis-inducing Ligand
Entrez Gene IDs
8743 (Human); 22035 (Mouse)
Alternate Names
Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10

Citations for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles
    Authors: M Frank-Bert, DS Pisetsky, C Kolling, BA Michel, RE Gay, A Jüngel, S Gay
    Front Immunol, 2018;9(0):28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Trail (TNF-related apoptosis-inducing ligand) induces an inflammatory response in human adipocytes
    Authors: V Zoller, JB Funcke, J Roos, M Dahlhaus, M Abd El Hay, K Holzmann, R Marienfeld, T Kietzmann, KM Debatin, M Wabitsch, P Fischer-Po
    Sci Rep, 2017;7(1):5691.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
    Sci Rep, 2016;6(0):36862.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The use of a stably expressed FRET biosensor for determining the potency of cancer drugs.
    Authors: Bozza, William, Di, Xu, Takeda, Kazuyo, Rivera Rosado, Leslie A, Pariser, Sarah, Zhang, Baolin
    PLoS ONE, 2014;9(9):e107010.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
    Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, Gregory
    PLoS ONE, 2014;9(3):e92124.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
    Authors: Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
    Biochem. J., 2009;420(1):73-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis.
    Authors: Roux S, Lambert-Comeau P, Saint-Pierre C, Lepine M, Sawan B, Parent JL
    Biochem. Biophys. Res. Commun., 2005;333(1):42-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2004;279(4):2937-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
    Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
    Exp. Cell Res., 2004;298(2):560-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
    Authors: Roybal K, Williams J, Morsut L, Rupp L, Kolinko I, Choe J, Walker W, McNally K, Lim W
    Cell, 0;167(2):419-432.e16.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Bioassay)

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Reviews for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

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Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein
By Anonymous on 12/16/2019
Application: Apoptosis assay