Recombinant Human Tripeptidyl-peptidase I/TPP1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)

Recombinant Human Tripeptidyl-peptidase I/TPP1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by the cleavage of Ala-Ala-Phe-AMC. The specific activity is >850 pmol/min/μg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Tripeptidyl-Peptidase I/TPP1 protein
Met1-Pro563, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
61 kDa
65-75 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

  • Activation Buffer: 50 mM NaOAc, 100 mM NaCl, pH 3.5
  • Assay Buffer: 50 mM NaOAc, 100 mM NaCl, pH 5.0
  • Recombinant Human Tripeptidyl‑Peptidase I/TPP1 (rhTPP1) (Catalog # 2237-SE)
  • Substrate: AAF-AMC (Bachem, Catalog # I-1415), 10 mM in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhTPP1 to 20 μg/mL in Activation Buffer.
  2. Incubate for 1 hour at 37 °C.
  3. Dilute rhTPP1 to 0.5 ng/μL in Assay Buffer.
  4. Dilute Substrate to 400 µM in Assay Buffer.
  5. Load into a black well plate 50 µL of the 0.5 μg/mL rhTPP1 and start the reaction by adding 50 µL of 400 µM substrate.
  6. Read at excitation and emssion wavelengths of 380 nm and 460 nM (top read), respectively, in kinetic mode for 5 minutes.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-methyl coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rhTPP1: 0.025 µg
  • Substrate: 200 µM
Reconstitution Calculator

Reconstitution Calculator

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Background: Tripeptidyl-Peptidase I/TPP1

Tripeptidyl-Peptidase I (TPP1), also known as tripeptidyl aminopeptidase, is a lysosomal peptidase which can hydrolyze tripeptides from the N-termini of oligopeptides and also possesses weak endopeptidase activity (1‑3). TPP1 is a serine peptidase with a Ser-Glu-Asp catalytic triad, making it a member of the sedolisin family (4). The TPP1 precursor undergoes autoactivation under conditions of acidic pH (4). TPP1 is expressed in many tissues, with elevated expression in tissues associated with peptide hormone production (5). Mutations in TPP1 have been shown to be a cause of classic late‑infantile neuronal ceroid lipofuscinosis (CLN2), a lysosomal storage disease (6).

  1. Sleat, D.E. et al. (1997) Science 277:1802.
  2. Ezaki, J. et al. (1999) J. Neurochem. 72:2573.
  3. Tomkinson, B. (1999) Trends Biochem. Sci. 24:355.
  4. Lin, L. et al. (2001) J. Biol. Chem. 276:2249.
  5. Kida, E. et al. (2001) J. Neuropathol. Exp. Neurol. 60:280.
  6. Sleat, D.E. et al. (1999) Am. J. Hum. Genet. 64:1511.         
Entrez Gene IDs
1200 (Human)
Alternate Names
Cell growth-inhibiting gene 1 protein; ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease); CLN2; CLN2EC; growth-inhibiting protein 1; LINCL; LPIC; lysosomal pepstatin insensitive protease; Lysosomal pepstatin-insensitive protease; MGC21297; TPP1; TPP-1; TPP-I; Tripeptidyl aminopeptidase; tripeptidyl peptidase I; tripeptidyl-peptidase 1; TripeptidylPeptidase I; Tripeptidyl-Peptidase I

Citations for Recombinant Human Tripeptidyl-peptidase I/TPP1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses
    Authors: N Sima, R Li, W Huang, M Xu, J Beers, J Zou, S Titus, EA Ottinger, JJ Marugan, X Xie, W Zheng
    Orphanet J Rare Dis, 2018;13(1):54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Intravitreal implantation of TPP1-transduced stem cells delays retinal degeneration in canine CLN2 neuronal ceroid lipofuscinosis
    Exp Eye Res, 2016;0(0):.
    Species: Human
    Sample Types:
    Applications: Enzyme Assay


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