Recombinant Human TrkA Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
175-TK-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (8)
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Recombinant Human TrkA Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.8-4 µg/mL in the presence of 10 ng/mL of rhNGF.
Source
Mouse myeloma cell line, NS0-derived human TrkA protein
Human TrkA
(Ala33-Glu407)
Accession #AAA36770
IEGRID Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
67.4 kDa (monomer)
SDS-PAGE
115-125 kDa, reducing conditions

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175-TK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

175-TK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

References
  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

Citations for Recombinant Human TrkA Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics
    Authors: AJ Poole, L Frigotto, ME Smith, C Baar, G Ivanova-Be, A Jaulent, C Stace, CG Ullman, AV Hine
    Sci Rep, 2019;9(1):930.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  2. Nerve Growth Factor Is Responsible for Exercise-Induced Recovery of Septohippocampal Cholinergic Structure and Function
    Authors: JM Hall, F Gomez-Pini, LM Savage
    Front Neurosci, 2018;12(0):773.
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo
  3. TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus
    Authors: JI Hurtado-Za, B Ramachandr, S Ahmed, R Halder, C Bolleyer, A Awasthi, MA Stahlberg, RJ Wagener, K Anderson, RM Drenan, HA Lester, JM Miwa, JF Staiger, A Fischer, C Dean
    Nat Commun, 2017;8(0):15878.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations
    Authors: JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  5. Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
    Authors: Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
    J Immunol, 2014;192(7):3345-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Brain-derived neurotrophic factor ameliorates brain stem cardiovascular dysregulation during experimental temporal lobe status epilepticus.
    Authors: Tsai CY, Chan JY, Hsu KS
    PLoS ONE, 2012;7(3):e33527.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: N/A
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  8. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: PcIP

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